海马神经元γ-氨基丁酸A受体在异氟醚导致新生大鼠认知功能障碍中的作用  

Role of gamma-aminobutyric acid A receptor in isoflurane-induced cognitive dysfunction in neonatal rats

作　　者：田海涛[1] 李海鸥[2] 王志萍[3] 

机构地区：[1]青岛大学医学院麻醉学系,266021 [2]山东省济宁市第一人民医院麻醉科 [3]南京医科大学附属无锡市人民医院麻醉科

出　　处：《中华麻醉学杂志》2012年第11期1347-1349,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价海马神经元γ-氨基丁酸A（GABAA）受体在异氟醚导致新生大鼠认知功能障碍中的作用。方法出生7dSD大鼠28只，雌雄不拘，体重16～18g，采用随机数字表法，将大鼠随机分为4组（n=7）：对照组（Con组）腹腔注射生理盐水0．2ml；一叶款碱组（Sec组）于相应时点腹腔注射GABA。受体特异性拮抗剂一叶蔌碱30mg／kg；异氟醚组（Iso组）腹腔注射生理盐水0．2ml，持续吸入1．4％异氟醚6h；一叶蔌碱＋异氟醚组（Sec＋Iso组）吸人异氟醚前30min和吸入3h时腹腔注射一叶蔌碱30mg／kg，持续吸入1．4％异氟醚6h。给药后3周时处死大鼠取海马制作海马切片，行电生理实验，每隔5min测定群峰电位振幅（PSA）和长时程增强（LTP），记录LTP诱发成功情况。结果与Con组比较，Is0组各时点PSA和LTP诱发成功率降低（P〈0．01），Sec组和Sec＋Iso组上述指标差异无统计学意义（P〉0．05）；与Is0组比较，Sec＋Iso组各时点PSA和LTP诱发成功率升高（P〈0．01）。结论GABA。受体参与了异氟醚导致新生大鼠认知功能障碍的过程。Objective To evaluate the role of gamma-aminobutyric acid A （GABAg ） receptor in isoflu- rane-induced cognitive dysfunction in neonatal rats. Methods Twenty-eight 7-day-old Sprague Dawley rats, weighing 16-18 g, were randomly divided into 4 groups （n = 7 each）： control group （group Con）, securinine group （group Sec）, isoflurane group （group Iso） and seeurinine ＋ isoflurane group （group See ＋ Iso） . Normal saline 0.2 ml was injected intraperitoneally in group Con. Specific GABAA receptor antagonist securinine 30 mg/kg was injected intraperitoneally at the corresponding time points in group Sec. Normal saline 0.2 ml was injected in- traperitoneally and 1.4% isoflurane was inhaled for 6 h in group Iso. Seeurinine 30 mg/kg was injected intraperito- neally at 30 min before isoflurane inhalation and 3 h of inhalation, and 1.4% isoflurane was inhaled for 6 h in group Sec ＋ Iso. The rats were then sacrificed at 3 weeks after administration, their brains were immediately re- moved and hippocampal slices were prepared for electrophysiological experiments. The value of population spike amplitude （PSA） and long-term potentiation （LTP） were measured every 5 min. The successful LTP induction was recorded. Results Compared with group Con, the values of PSA and rates of successful LTP induction were sig- nificantly decreased in group Iso （P 〈 0.01 ）, and no significant change was found in the parameters mentioned above in groups See and Sec ＋ Iso （ P 〉 0.05） . The values of PSA and rates of successful LTP induction were sig- nificantly higher in group Sec ＋ Iso than in group Iso （ P 〈 0.01 ）. Conclusion GABAA receptor is involved in isoflurane-induced cognitive dysfunction in the neonatal rats.

关 键 词：受体 GABA 异氟醚 认知障碍 婴儿 新生 

分 类 号：R726.1[医药卫生—儿科]