芬太尼、舒芬太尼和瑞芬太尼对人脐血树突状细胞免疫功能影响的比较  

Comparison of effects of fentanyl, sufentanil and remifentanii on immune function of dendritic cells in hu- man umbilical cord blood

作　　者：邢玉英[1] 齐少霞[2] 赵雪莲[1] 付建峰[1] 

机构地区：[1]河北医科大学第四医院麻醉科,石家庄市050011 [2]河北省沧州中西医结合医院麻醉科

出　　处：《中华麻醉学杂志》2012年第11期1363-1366,共4页Chinese Journal of Anesthesiology

基　　金：基金项目：河北省普通高等学校强势特色学科肿瘤学建设经费资助项目（2005-52）

摘　　要：目的比较芬太尼、舒芬太尼和瑞芬太尼对人脐血树突状细胞免疫功能的影响。方法密度梯度离心法分离人脐血单个核细胞，以1．0×10^6个／ml密度接种于24孔培养板中，2ml／孔，采用随机数字表法，将人脐血单个核细胞随机分为7组（n=15）：正常对照组（C组）、1．0ng／ml芬太尼组（F．组）、5．0ng／ml芬太尼组（F5组）、0．1ng／ml舒芬太尼组（s，组）、0．5ng／ml舒芬太尼组（S5组）、1．0ng／ml瑞芬太尼组（R，组）、5．0ng／ml瑞芬太尼组（R5组）。7组先用含有50ng／ml重组人粒细胞集落刺激因子、10ng／ml重组人白细胞介素．4和相应浓度的芬太尼、舒芬太尼或瑞芬太尼的无血清培养基孵育10d，然后加入50ng／ml重组人肿瘤坏死因子-α继续孵育4d。每组取3孔，倒置相差显微镜观察细胞形态学，每组取6孔，测定培养液IL-12浓度和CD80／CD86表达。每组取6孔，测定树突状细胞活力。结果与C组比较，R组、S1组、S5组、R1组和R5组培养液IL-12浓度和细胞活力降低，CD80／CD86表达下调（P〈0．05），F1组上述指标差异无统计学意义（P〉0．05）；与FI组比较，S1组和R1组培养液IL-12浓度和细胞活力降低，CD80／CD86表达下调（P〈0．05）；与R组比较，S5组培养液IL-12浓度降低（P〈0．05），细胞活力和CD80／CD86表达差异无统计学意义（P〉0．05），R5组培养液IL-12浓度和细胞活力降低，CD80／CD86表达下调（P〈0．05）；与S5组比较，R5组培养液IL-12浓度和细胞活力降低（P〈0．05），CD80／CD86表达差异无统计学意义（P〉0．05）；与S5组比较，R5组培养液IL-12浓度和细胞活力降低，CD80／CD86表达下调（P〈0．05）。结论瑞芬太尼对人脐血树突状细胞免疫功能的抑制作用强于舒芬太尼，舒芬太尼对其抑制作用强于芬太尼。Objective To compare the effects of fentanyl, sufentanil and remifentanil on the immune function of dendritic cells in human umbilical cord blood. Methods Human umbilical cord blood mononuclear cells were obtained by density gradient centrifugation and seeded in 24-well plates with a density of 1 × 10^6/ml （2 ml/hole）. The cells were randomly divided into 7 groups （ n = 15 each） ： control group （group C）, fentanyl 1.0 ng/ml group （group F1 ）, fentanyl 5.0 ng/ml group （group F5 ）, sufentanil 0.1 ng/ml group （group S1 ）, sufentanil 0.5 ng/ml group （group S5 ）, remifentanil 1.0 ng/ml group （group R1 ）, and remifentanil 5.0 ng/ml group （group R5 ）. The cells were incubated for 10 days in serum-free culture medium containing 50 ng/ml recombinant human granulocyte colony stimulating factor, 10 ng/ml recombinant human interleukin-4 or the corresponding concentration of fentanyl, sufentanil or remifeutanil, and then 50 ng/ml recombinant human tumor necrosis factor alpha was add- ed to the culture medium and the cells were incubated for another 4 days in the seven groups. Three holes in eachgroup were chosen and the cell morphology was examined with inverted microscope. Six holes in each group were chosen for determination of the concentration of IL-12 in the supematant and expression of CD80/CD86. Six holes in each group were chosen for measurement of the cell viability. Results Compared with group C, the concentra- tion of IL-12 and cell viability were significantly decreased and the expression of CD80/CD86 was down-regulated in groups F5 , S1 , S5, R1 and R5 （P 〈 0.05）. The coficentration of IL-12, cell viability and expression of CD80/ CD86 were significantly lower in groups S1 and R1 than in group F1 （P 〈 0.05）. Compared with group F5 , the concentration of IL-12 was significantly decreased in group S5 , and the concentration of IL-12 and cell viability were significantly decreased and the expression of CD80/CD86 was down-regulated in group R5 （P 〈 0.05 ）. The

关 键 词：芬太尼 舒芬太尼 哌啶类 免疫 细胞 树突细胞 

分 类 号：R614[医药卫生—麻醉学]