异氟醚预处理对大鼠局灶性脑缺血再灌注时5脂氧合酶表达的影响  

Effect of isoflurane preconditioning on expression of 5-1ipoxygenase during focal cerebral ischemia-reperfusion in rats

作　　者：吕海港[1] 任鹏程[1] 高昌俊[1] 孙美艳[1] 赵晓勇[1] 柴伟[1] 孙绪德[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,西安市710038

出　　处：《中华麻醉学杂志》2012年第11期1383-1386,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972833）

摘　　要：目的探讨异氟醚预处理对大鼠局灶性脑缺血再灌注时5脂氧合酶（5-LOX）表达的影响。方法雄性成年sD大鼠39只，体重250～300g，采用随机数字表法，将大鼠随机分为3组（n=13）：假手术组（S组）、局灶性脑缺血再灌注组（I／R组）和异氟醚预处理组（I组）。采用大脑中动脉阻断法制备大鼠局灶性脑缺血再灌注模型。I组吸入2％异氟醚2h，24h后制备模型。再灌注24h时进行神经功能缺陷评分，随后处死，取脑组织钡4量脑梗死体积，分别采用Westernblot法和RT—PCR法测定5-LOX、髓样分化因子88（MyD88）和NF＋KB蛋白及其mRNA的表达水平。结果与s组比较，I／R组和I组神经功能缺陷评分升高，脑梗死体积增大，I／R组5-LOX、MyD88和NF—xB蛋白及其mRNA表达上调，I组5-LOXmRNA、MyD88蛋白及其mRNA表达上调（P〈0．05）；与I／R组比较，I组神经功能缺陷评分降低，脑梗死体积减小，5-LOX、MyD88和NF．wB蛋白及其mRNA表达下调（P〈0．05）。结论异氟醚预处理可能通过下调5-LOX表达，抑制MyD88／NF．xB信号通路，从而减轻大鼠局灶性脑缺血再灌注损伤。Objective To investigate the effect of isoflurane preconditioning on the expression of 5-1ipoxy- genase （5-LOX） during focal cerebral ischemia-reperfusion （I/R） in rats.Methods Thirty-nine male adult Spra- gue-Dawley rats weighing 250-300 g were randomly divided into 3 groups （ n =13 each） ： sham operation group （group S）; focal cerebral I/R group （group I/R）; isoflurane preconditioning group （group I）. Focal cerebral I/R was produced by mid-cerebral artery occlusion in anesthetized rats. The rats inhaled 2 h of 2% isoflurane and focal cerebral I/R was produced 24 h later in group I. The neurological deficits were scored at 24 h of reperfusion. The animals were then sacrificed. The brains were immediately removed for determination of the infarct size. The ex- pression of 5-LOX, myeloid differentiation factor88 （MyD88） and nuclear factor kappa B （NF-~B） protein and mRNA was detected using Western blot and RT-PCR respectively. Results Compared with group S, the neurologi- cal deficit score was significantly increased, the infarct size was enlarged in groups I/R and I, the expression of 5- LOX, MyD88 and NF-tcB protein and mRNA was up-regulated in group I/R, and the expression of 5-LOX mRNA and MyD88 protein and mRNA was up-regulated in group I （ P 〈 0.05）. Compared with group I/R, the neurologi- cal deficit score was significantly lower, the infarct size was smaller, and the expression of 5-LOX, MyD88 and NF-κB protein and mRNA was lower in group I （ P 〈 0.05 ） . Conclusion Isoflurane preconditioning can reduce focal cerebral I/R injury by down-regulating the expression of 5-LOX and inhibiting MyD88/NF-tcB signaling path- way in rats

关 键 词：花生四烯酸盐5-脂氧合酶 异氟醚 缺血预处理 再灌注损伤 脑 

分 类 号：R965[医药卫生—药理学]