凋亡相关蛋白HSP90α和Clusterin在自身免疫性甲状腺疾病中的表达  

Expression of apoptosis-related protein HSP90α, Clusterin in autoimmune thyroid diseases

作　　者：赵艳利[1] 张会娟[2] 陈景斌[1] 

机构地区：[1]郑州大学附属郑州中心医院内分泌科,河南郑州450007 [2]郑州大学第一附属医院内分泌科,河南郑州450052

出　　处：《中国现代医学杂志》2012年第32期51-55,共5页China Journal of Modern Medicine

摘　　要：目的探讨凋亡相关蛋白HSP90α、Clusterin的表达与自身免疫性甲状腺疾病发生发展的关系。方法应用免疫组织化学SP法检测26例Graves病(GD)、15例桥本甲状腺炎(HT)患者甲状腺组织标本和11例正常甲状腺组织中HSP90α、Clusterin的表达,并分析两者之间的相关性。结果 HSP90α在甲状腺滤泡上皮细胞胞质、胞核均有表达。HSP90α在GD、HT均以胞浆表达为主。Clusterin在GD、HT主要表达在甲状腺滤泡上皮细胞的胞浆和胞膜。GD和HT中HSP90α、Clusterin的表达均明显高于正常甲状腺组织。结论HSP90α和Clusterin在GD中异常高表达可能主要通过抑制部分甲状腺细胞凋亡,间接促进甲状腺组织的增生;HSP90α和Clusterin在HT中甲状腺滤泡上皮的高表达可能是机体在病理情况下的一种代偿现象,能阻止部分甲状腺细胞凋亡,对机体产生保护作用。HSP90α、Clusterin的表达可能在自身免疫性甲状腺疾病(AITD)的发生发展过程中起重要作用。【Objective】 To investigate the relationship between the expression of apoptosis-related protein HSP90α, Clusterin and the initiation and / or maintenance of autoimmune thyroid diseases. 【Methods】 SP immunohistochemical method was performed to detect the expression of HSP90α, Clusterin in 26 cases of Graves disease, 15 cases of Hashimoto thyroiditis and 11 cases of normal thyroid tissues. The correlation between the expression of HSP90α and Clusterin in Graves disease and Hashimoto thyroiditis was also analyzed. 【Results】 HSP90α was localized in the cytoplasm, nuclei of thyroid follicle epithelial cells. HSP90α was mainly localized in the cytoplasm in Graves disease, Hashimoto thyroiditis. In Graves disease and Hashimoto thyroiditis, Clusterin was mainly localized in the membrane and cytoplasm of thyroid follicle epithelial cells. The expression of HSP90α and Clusterin was significantly higher than that in normal thyroid tissues. 【Conclusion】 HSP90α and Clusterin were mainly secreted by thyroid follicle epithelial cells. In Hashimoto thyroiditis. The highly expressed HSP90α, Clusterin may inhibit the apoptosis of thyroid follicle epithelial cells in Graves disease and cause diffuse goiter and hyperfunction. In Hashimoto thyroiditis, the highly expressed HSP90α, Clusterin can protect thyroid tissues by inhibiting the apoptosis of thyrocytes. The expression of HSP90α and Clusterin may play an important role in the initiation and / or maintenance of autoimmune thyroid diseases.

关 键 词：格雷夫斯病 桥本甲状腺炎 HSP90Α CLUSTERIN 免疫组织化学 

分 类 号：R581[医药卫生—内分泌]