肝郁脾虚证大鼠双侧海马CA1区注射AMPA后行为的变化及逍遥散的调节作用  

Behavior changes after injection of AMPA in bilateral hippocampus CA1 zone in rats with syndrome of liver depression and spleen deficiency and regulative effect of Xiaoyao San

作　　者：岳利峰[1] 王玲[2] 赵健[1] 陈昌乐[1] 奚胜艳[3] 王琦 

机构地区：[1]北京中医药大学北京100029 [2]北京航空航天大学 [3]厦门大学 [4]山西省实验中学

出　　处：《北京中医药大学学报》2012年第12期817-821,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学青年基金项目(No.61101008);北京中医药大学自主选题项目(No.2009JYBZZ-JS006)

摘　　要：目的从行为学角度进一步探讨逍遥散治疗肝郁脾虚证的调节机制。方法 75只SD雄性大鼠随机分为5组,正常组、模型组、假手术组、α-氨基羟甲基恶唑丙酸(AMPA)组和逍遥散组。以21 d慢性束缚应激方法造肝郁脾虚证模型,于模型大鼠双侧海马CA1区,埋管微量注射AMPA受体的激动剂AMPA,逍遥散组造模方法和AMPA组尽可能相似,突出逍遥散和AMPA 2种干预的可比性。于第1、7、14、21 d分别比较AMPA组和逍遥散组行为变化各项指标趋势是否一致。结果模型组大鼠逐步呈现肝郁脾虚证表现,AMPA组、逍遥散组大鼠焦躁状态逐步得到抑制。造模第21天,与正常组比较,AMPA组和逍遥散组大鼠穿格次数、站立次数、修饰次数等指标均无统计学意义(P>0.05)。结论从宏观行为学角度,初步推断逍遥散可能通过纠正杏仁核和海马的"兴奋-抑制"失衡,重建稳态,来治疗肝郁脾虚证。Objective To probe further into the regulative mechanism of Xiaoyao San in the treatment of syndrome of liver depression and spleen deficiency from the angle of ethology. Methods Male SD rats （n = 75） were randomly divide into normal group, model group, sham-operation group, AMPA group and Xiaoyao San group. The model of syndrome of liver depression and spleen deficiency was established with 21-day chronic constraint stress and inicroinjection of AMPA in CA1 zone of bilateral hippocampus. The modeling method in Xiaoyao San group was as the same as possible with that in AMPA group for highlighting the comparability between Xiaoyao San and AMPA. The tendency of all ethological indexes was compared between AMPA group and Xiaoyao San group respectively on the 1st, 7th, 14th and 21st day. Results The model group gradually showed the manifestations of syndrome of liver depression and spleen deficiency. The restless status was gradually inhibited in AMPA group and Xiaoyao San group. On the 21st day, the times of passing, and Xiaoyao San group compared standing and making up had no statistical significance in AMPA group with normal group, （ all P 〉 0.05 ）. Conclusion From the angle ofmacro-ethology, it can be initially deduced and spleen deficiency through correcting the that Xiaoyao San can treat the disorder of excitation-inhibition syndrome of liver depression and setting up again a stable status in amygdale and hippocampus.

关 键 词：逍遥散 行为学 肝郁脾虚证 调节机制 α-氨基羟甲基恶唑丙酸 大鼠 

分 类 号：R285.5[医药卫生—中药学]