何首乌的抗抑郁作用及其对海马5HT_(1A)受体表达和神经细胞发生的影响  

Anti-depression effect of Heshouwu (Radix Polygoni Muotiflori) and its influences on expression of hippocampus 5HT1A receptor and neurogenesis

作　　者：畅洪昇[1] 鲁艺[1] 王伟明[1] 李丽娜[1] 孙文燕[1] 王伟[1] 王庆国[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2012年第12期822-825,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2011CB505101)

摘　　要：目的研究养血补肝药物何首乌的抗抑郁作用和机制。方法采用束缚联合慢性不可预知温和应激大鼠、小鼠抑郁模型,通过敞箱行为和蔗糖水偏嗜度测试评价何首乌的抗抑郁药效作用。通过RT-PCR技术分析海马5HT1A基因表达,通过腹腔注射溴脱氧核苷尿嘧啶(Brdu)标记新生神经细胞,并以酶联免疫(ELISA)检测分析脑组织神经细胞发生。结果何首乌(2 g/kg)增加了慢性应激大鼠的水平运动、蔗糖水偏嗜度(P<0.05),以及海马5HT1AmRNA表达(P<0.01)。何首乌(2.85 g/kg)增加了慢性应激小鼠脑组织中Brdu含量(P<0.01),以上作用与氟西汀相似。结论何首乌可以改善慢性应激动物的抑郁行为,其机制可能与改善5HT能神经元传递,增加中枢神经细胞增殖等有关。Objective To study the anti-depression effect and mechanism of Heshouwu （Radix Polygoni Muotiflori） , a medicinal with the actions of nourishing blood and tonifying liver. Methods The rat and mouse models of depression were established by using bounding combining chronic unpredictable mild stress. The anti-depression effect of Heshouwu was evaluated through open field test and sucrose preference degree detection. The expression of 5HT1A was analyzed by using RT-PCR technique. New neurons were labeled through the intraperitoneal injection of Brdu, and neurogenesis in brain tissue was detected by applying ELISA. Results Heshouwu （2 g/kg ） improved the horizontal movement and sucrose preference degree （ P 〈 0.05 ） , and increased the expression of hippocampus 5HTtA mRNA in chronic stress rats. Heshouwu （2.85 g/kg） increased the content of brain Brdu in chronic stress mice （ P 〈 0.01 ）. All above effects of Heshouwu were similar to those of fluoxetine. Conclusion Heshouwu can relieve the depression behaviors of chronic stress animals, and the mechanism may be related to that it can improve 5HT neuron transmission and axoneuron proliferation. The therapy of nourishing blood and tonifying liver and related medicinal and formulas is important in the treatment of depression.

关 键 词：何首乌 抗抑郁 5HT1A受体 神经细胞发生 

分 类 号：R285.5[医药卫生—中药学]