新疆两种纤维亚麻胚轴段高频萌芽诱导  

High Frequency Adventitious Budding Induction from Hypocotyls Segments of Two Kinds of Fiber Flax in Xinjiang

作　　者：邓倩[1] 刘静文[1] 王伟[1] 计巧灵[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《新疆农业科学》2012年第12期2280-2285,共6页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目"纤维亚麻体细胞耐盐突变体的筛选"(31160295)

摘　　要：【目的】通过建立新疆主栽的两个纤维亚麻品种（范妮和天鑫3号）高频率不定芽离体再生体系，为进一步筛选其体细胞耐盐突变体奠定基础。【方法】以不同消毒剂处理种子，以下胚轴段为外植体，运用不同植物生长调节剂组合诱导胚轴段再生不定芽。【结果】两种种子在10％的H2O2中处理10min灭菌效果最好；范妮不定芽诱导最适宜培养基为：MS＋BA0．45mg／L＋IAA0．3mg／L，不定芽诱导率达180％；天鑫3号不定芽诱导最适培养基为：MS＋BA0．6mg／L＋NAA0．03mg／L，不定芽的诱导率达到98％。【结论】不同的亚麻品种在下胚轴萌芽过程中对植物生长调节剂的配比和浓度的要求也不尽相同，探明了范妮和天鑫3号高频不定芽发生的最佳植物生长调节剂的组合和浓度。[ Objective] The purpose of this study was to lay the foundation for further screening of somatic salt tolerant mutants through establishment of high frequency in vitro regeneration system of adventitious buds of two species of flax （ Fany and Tianxin 3 ） that are mainly planted in Xinjiang. [ Method ] The seeds were sterilized with different disinfectants. With hypocotylar segments of the seeding as explants, regenerated adventitious buds in segments were induced by different combination and concentration of plant growth regula tots. [Result] Sterilizing effects were more suitable for the two kinds seeds treated with 10% H2O2 for 10 min. The optimum medium for inducing adventitious buds from Fany hypocotyls was MS ＋ BA 0.45 mg/L ＋ IAA 0. 3 mg/L, induction rate of adventitious bud reached 180%. The optimum medium for inducing adventi- tious buds from Tianxin 3 hypocotyls was MS ＋ BA 0.6 mg/L ＋ NAA 0. 03 mg/L, induction rate of adventitious bud reached 98 %. [ Conclusion ] The results showed that different species of flax need different combination and concentration of plant growth regulators for inducing adventitious buds from hypocotyls, and the optimum combination and concentration of plant growth regulators for inducing adventitious buds of Fany and Tianxin 3 hypocotyls were explored through tests.

关 键 词：亚麻 灭菌方法 下胚轴段 不定芽 

分 类 号：S563.2[农业科学—作物学]