G—CSF、AMD3100对大鼠间充质干细胞增殖、迁移和黏附能力影响的研究  

Study on effects of G-CSF and AMD3100 on proliferation, migration and adhesion of rat mesenchymal stem cells

作　　者：董晖[1] 余勤[1] 刘丽珍[2] 王艳[3] 宣晓波[3] 王标[1] 单威[1] 周丽萍[1] 刘伟[1] 

机构地区：[1]浙江中医药大学生物工程学院,杭州310053 [2]浙江大学医学院附属第一医院,杭州310003 [3]浙江中医药大学第一临床医学院,杭州310053

出　　处：《国际生物医学工程杂志》2012年第6期343-345,349,I0006,共5页International Journal of Biomedical Engineering

基　　金：浙江省科技厅项目（2010C33024）;浙江省新苗人才计划项目（2011R410037）;浙江省自然科学基金项目（LY12C10001）

摘　　要：目的探讨粒细胞集落刺激因子（G—CSF）、AMD3100对大鼠间充质干细胞（MSCs）增殖、迁移和黏附的影响。方法采用MTT比色法、Transwell迁移法和黏附试验分别检测体外G—CSF、AMD3100对间充质干细胞的增殖、迁移和黏附能力的影响。结果在G—CSF质量浓度为200μg／L、AMD3100质量浓度为0．5mg／L时MSCs增殖能力最强；在G-CSF质量浓度为200μg／L、AMD3100质量浓度为0mg／L时MSCs迁移能力最强；在G-CSF质量浓度为200μg／L、AMD3100质量浓度为0．5mg／L时MSCs黏附能力最强。结论体外G—CSF对间充质干细胞的增殖、迁移和黏附能力呈促进作用，而AMD3100呈抑制作用。Objective Tostudytheeffectsofgranulocytecolony-stimulatingfactor（G-CSF）andAMD3100on the proliferation, migration and adhesion of mesenchymal stem cells （MSCs）. Methods The proliferation, migration and adhesion of MSCs were detected by MTT chromometry, transwell and adhesion test. Results When the concentration of G-CSF was 200 μg/L and AMD3100 was 0.5 rag/L, the proliferation and migration of MSCs were the strongest. When the concentration of G-CSF was 200 μg/L and AMD3100 was 0 mg/L, the adhesion of MSCs was the strongest. Conclusion The proliferation, migration, adhesion of MSCs are promoted by G-CSF, and inhibited by AMD3100.

关 键 词：粒细胞集落刺激因子 AMD3100 间充质干细胞 增殖 迁移 黏附 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]