RGD多肽化学修饰聚苯乙烯培养板的细胞相容性研究  

Study on the cell compatibility of polystyrene plate chemically modified by RGD peptides

作　　者：王海[1] 梁士民[2] 刘兰霞[1] 庞丽云[1] 张超[1] 葛泉波[1] 朱敦皖[1] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所 天津市生物医学材料重点实验室,天津300192 [2]天津华立达生物工程有限公司,300457

出　　处：《国际生物医学工程杂志》2012年第6期357-360,364,F0003,共6页International Journal of Biomedical Engineering

基　　金：国家自然科学基金青年科学基金项目（81100100）;天津市自然科学基金面上项目（10JCYBJC10600）;教育部新教师基金项目（20091106120048）

摘　　要：目的考察RGD多肽化学修饰聚苯乙烯培养板细胞相容性研究。方法采用高锰酸钾硫酸溶液对聚苯乙烯表面进行氧化反应，生成羧基位点；水溶性碳二亚胺活化羧基，接枝明胶、胶原和RGD多肽。用红外光谱、X-射线光电子能谱（XPS）、动态接触角等研究了RGD化学修饰聚苯乙烯的变化，并考察了RGD化学修饰聚苯乙烯后细胞相容性的变化。结果XPS结果证实了聚苯乙烯表面N原子的引入，RGD是共价键合在聚苯乙烯表面的。动态接触角下降显著，证明修饰表面的亲水性能提高。在修饰后的聚苯乙烯培养板种植人表皮细胞，结果显示提高了细胞的黏附和增殖能力。结论聚苯乙烯表面进行RGD化学修饰，有利于提高细胞在其表面的黏附和增殖能力，有望为免疫磁珠分离得到的高纯度内皮祖细胞提供良好的培养介质。Objective To investigate the cell compatibility of polystyrene（PS） plate chemically modified with RGD peptides. Methods PS surfaces were carboxylated by permanganate oxidation in diluted sulfuric acid, and carboxyls were activated with water-soluble carbodiimide to graft with gelatin, collagen and RGD peptides. IR, X-ray photo-electronic spectroscopy （XPS） and dynamic contact angle were used to characterize the surface modification of PS surface. Results XPS results confirmed the existence of nitrogen element from protein molecules and the covalently binding of proteins to PS surfaces. Dynamic contact angle measurement indicated hydrophilicity of PS surfaces was improved obviously after grafting modification. The cell culture results showed that the cell adhesion and proliferation was better on modified surfaces than the initial. Conclusion The cell compatibility of PS surface was great improved after modification with RGD peptides, which would provide a potential strategy to improve the culture of purified endothelial progenitor ceils isolated by immunomagnetic beads.

关 键 词：RGD多肽 聚苯乙烯 细胞相容性 内皮祖细胞 

分 类 号：R318.08[医药卫生—生物医学工程]