电子克隆技术及其在医学领域的应用  

Technique of silico cloning and its application in medicine

作　　者：王朝辉[1] 马小兵[1] 

机构地区：[1]河北联合大学基础医学院,唐山063000

出　　处：《国际生物医学工程杂志》2012年第6期369-372,共4页International Journal of Biomedical Engineering

基　　金：河北省科学技术研究与发展计划项目（09276192D）

摘　　要：基于表达序列标签（ESTs）和基因组数据库的电子克隆（insilico cloning）策略，是近年发展起来的一门基因克隆的新技术。其技术核心是利用生物信息学技术组装延伸ESTs序列，获得基因部分乃至全长cDNA序列。它具有投入低、速度快、技术要求低和针对性强等优点。人类疾病发生与基因的关系已经被广泛证实，因此电子克隆技术必将成为疾病研究的重要手段。旨在阐述电子克隆技术在医学领域的应用进展。The strategy of silico cloning based on the express sequence tags sequence （ESTs）and genome database is a new technique developed in recent years, and the core of the technique is to use bioinformatics technique to assemble and extend ESTs to get part of eDNA and even all sequence. It has the virtue of low investment, high speed, low and well-targeted technical requirements, etc.The relationship between human disease and genes has been widely confirmed, therefore, silico cloning technology will become the important means of disease research. This paper aims at elaborating the progress of silico cloning in medicine.

关 键 词：表达序列标签 电子克隆 生物信息学 基因克隆 

分 类 号：Q943.2[生物学—植物学]