当归-川芎药对不同制法与配比抗氧化活性相互作用研究  

Study on antioxidant interaction of different preparations and proportions of Danggui-Chuanxiong drug pair

作　　者：黄美艳[1] 唐于平[1] 尚尔鑫[1] 郭建明[1] 刘欣[1] 王林艳[1] 李伟霞[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210046

出　　处：《中国中药杂志》2013年第2期234-238,共5页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2008BAI51B01);高等学校博士学科点专项科研基金(20113237110010);江苏省高校自然科学重大基础研究项目(06KJA36022;10KJA360039);国家教育部"新世纪优秀人才支持计划"(NCET-09-0163);江苏高等学校优秀科技创新团队支持计划(2009年度);江苏高校优势学科建设工程项目(ysxk-2010)

摘　　要：目的:应用响应曲面分析法观察归芎药对不同制法、不同配比对DPPH自由基清除率体外抗氧化活性的相互作用研究。方法:采用2,2-二苯基-1-苦肼基(DPPH)自由基清除率法测定当归-川芎10个配比,3种不同提取方法的提取物的抗氧化活性,应用非线性回归的方法确定量效曲线的各个参数,建立三维响应面模型,应用Matlab软件构建响应曲面的三维图形。结果:不同配比不同制法的30个样品,均具有明显的清除自由基活性,且表现出明显的量效关系。水提液的协同作用范围窄,几乎成点状分布,拮抗作用范围呈6条宽窄不一的条带状分布;醇提液的协同作用呈小条状分布,拮抗作用呈点状分布。在先水提后醇提液协同作用呈条状分布(3条),拮抗作用呈点状分布。总之先水提后醇提液的协同作用区域大于醇提液,水提液次之;3种提取方法大部分区域均未表现出明显协同与拮抗作用。结论:采用响应曲面分析法定量研究中药配伍药物相互作用,为相关研究提供了思路与方法的参考。Objective： To observe the in vitro antioxidant interaction of different preparations and proportions of Danggui-Chua- nxiong drug pair in the DPPH free radical scavenging rate with the response surface methodoh）gy. Method： The 2,2-diphenyI-I-pieryl- hydrazyl （DPPH） free radical scavenging rate method was adopted for determining the antioxidant activity of extracts from Danggui- Chuanxiong with 10 proportions and three extraction processes. The response surtace methodology was used to determine the parameters of the dose-effect curve and establish a three-dimensional response surface model. The three-dimensional response surface graph was construeted with Matlab software. Result： All of the 30 samples with different proportions and preparations had antioxidant effect in scavenging free radicals and a remarkable dose-effect relationship. Their water extracts had a narrow synergistic range, with only spot distribution. Their antagonist ranges were districted in six bands of various widths. The synergistic ranges of ethanol extracts were dis- tricted in small bands, with the antagonist ranges scattered in points. The synergistic ranges of their water-alcohol extracts were distrib- uted in three bands, with their antagonist ranges scattered in points. In short, the water-alcohol extracts showed a wider synergistic range than ethanol extracts, followed by water-extracts. All of the three extraction processes showed no obvious synergistic and antago- nist effects. Conclusion： The quantitative study on the interaction of traditional Chinese medicines with different compatibilities with the response suffaee methodology provides reference of thoughts and methods for relevant studies.

关 键 词：当归 川芎 药对 抗氧化 DPPH 响应曲面分析法 相互作用 

分 类 号：R285[医药卫生—中药学]