濒危药用植物桃儿七野生居群遗传多样性与遗传结构的SCoT分析  

Genetic diversity and genetic structure of endangered wild Sinopodophyllum emodi by start codon targeted polymorphism

作　　者：陈大霞[1] 赵纪峰[1] 刘翔[1] 王昌华[1] 张植伟[1] 秦松云[1] 钟国跃[2] 

机构地区：[1]重庆市中药研究院濒危药材繁育国家工程实验室,重庆400065 [2]江西中医学院,江西南昌330004

出　　处：《中国中药杂志》2013年第2期278-283,共6页China Journal of Chinese Materia Medica

基　　金：全国生物物种资源联合执法检查和调查项目(ZYS-428-0908)

摘　　要：目的:揭示珍稀濒危药用植物桃儿七的遗传多样性水平及居群遗传结构特点。方法:采用新型分子标记SCoT对来源于我国的6个桃儿七野生居群共45个个体进行遗传多态性检测。运用POPGENE软件计算相关遗传参数,UPGMA方法聚类,结合MEGA5软件生成树状图。结果:筛选出的27条引物,共检测到350个位点,其中284个为多态位点,平均每条引物扩增所得多态条带为10.52条。物种水平上,桃儿七6个野生居群的遗传多样性较为丰富,PPB 79.27%,Ne1.332 7,H 0.210 9,Hsp0.328 6。在居群水平上,遗传多样性水平较低:PPB 10.48%(4.00%～23.71%),Ne1.0487(1.020 7～1.1037),H 0.029 7(0.012 9～0.063 1),Hpop0.046 2(0.019 9～0.098 6)。Nei's基因多样性指数计算的居群间遗传分化系数Gst=0.841 1与Shannon's居群分化系数(Hsp-Hpop)/Hsp=0.849 4基本一致,均说明大部分遗传变异存在于居群间。基因流Nm=0.094 4,表明桃儿七居群间基因交流处于低等水平。Nei's遗传一致度(I)范围为0.570 8～0.978 7。聚类分析将供试居群分为2类,相同地理来源或相似生境的材料具有聚为一类的倾向。结论:供试野生桃儿七具有较丰富的遗传多样性,为有效保护和改良种质资源奠定了一定的基础。Objective： Revealed the genetic diversity level and genetic structure characteristics in Sinopodophyllum emodi, a rare and endangered species in China. Method： We detected the genetic polymorphism within and among six wild populations （45 in- dividuals） by the approach of Start Codon Targeted （SCOT） Polymorphism. The associated genetic parameters were calculated by POP- GENE1.31 and the relationship was constructed based on UPGMA method. Result： A total of 350 bands were scored by 27 primers and 284 bands of them were polymorphic. The average polymorphic bands of each primer were 10. 52. At species level, there was a high level of genetic diversity among six populations （ PPB = 79. 27%, No = 1. 332 7, H = 0. 210 9 and H,p = 0. 328 6）. At population level, the genetic diversity level was low （ PPB = 10. 48% （4. 00% -23.71% ）, N, = 1. 048 7 （ 1. 020 7-1. 103 7 ）, H = 0. 029 7 （0. 012 9-0. 063 1）, Hpop =0. 046 2（0. 019 9-0. 098 6）. The Nei＇s coefficient of genetic differentiation was 0. 841 1, which was con- sistent with the Shannon＇s coefficient of genetic differentiation （0. 849 4）. Two calculated methods all showed that most of the genetic variation existed among populations. The gene flow （ Nm = 0. 094 4） was less among populations, indicating that the degree of genetic differentiation was higher. Genetic similarity coefficient were changed from 0. 570 8 to 0. 978 7. By clustering analysis, the tested pop- ulations were divided into two classes and had a tendency that the same geographical origin or material of similar habitats clustered into one group. Conclusion： The genetic diversity of samples of S. emodi is high, which laid a certain foundation for effective protection and improvement of germplasm resources.

关 键 词：桃儿七 SCoT 遗传多样性 遗传结构 

分 类 号：R29[医药卫生—民族医学]