枸橼酸芬太尼对人乳腺癌MCF-7细胞MMP-2及MMP-9表达的影响  

Effect of fentanyl on expressions of matrix memlloproteinase-2 and matrix memlloproteinase-9 in human breast cancer MCF-7 cells

作　　者：赵海燕 张宏波[2,3] 张海生 曹文波[3] 

机构地区：[1]河南省郑州市第九人民医院麻醉科,450053 [2]郑州大学基础医学院解剖学教研室 [3]郑州大学基础医学院基础肿瘤学教研室

出　　处：《中国妇幼保健》2013年第3期525-527,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨枸橼酸芬太尼对人乳腺癌MCF-7细胞MMP-2和MMP-9表达的影响。方法:以MCF-7细胞为对象,分别加入0、0.000 1、0.01、1μmol/L的枸橼酸芬太尼干预48 h,RT-PCR检测MMP-2和MMP-9 mRNA的表达,Western blot检测MMP-2和MMP-9蛋白的表达。结果:MCF-7细胞MMP-2和MMP-9 mRNA的表达随着枸橼酸芬太尼浓度的增加而减少,0.01及1μmol/L枸橼酸芬太尼组与对照组差异有统计学意义(P<0.05)。枸橼酸芬太尼呈浓度依赖性抑制MCF-7细胞MMP-2、MMP-9蛋白的表达,各浓度实验组与对照组差异有统计学意义(P<0.01)。结论:芬太尼可抑制MCF-7细胞MMP-2及MMP-9的表达,该作用可能是芬太尼降低人乳腺癌MCF-7细胞侵袭能力的分子机制之一。Objective：To explore the effect of fentanyl on expressions of matrix memlloproteinase-2（MMP-2） and matrix memlloproteinase-9（MMP-9） in human breast cancer MCF-7 cells in vitro.Methods：MCF-7 cells were treated respectively with various concentrations of fentanyl for 48 hours.Reverse transcription polymerase-chain reaction（RT-PCR） was used to examine the expressions of MMP-2 and MMP-9 mRNA.Western blot was used to evaluate the productions of MMP-2 and MMP-9 proteins.Results：The expressions of MMP-2 and MMP-9 mRNA in MCF-7 cells decreased after treated with fentanyl.There was statistically significant difference between 0.01 μmol/L,1 μmol/L fentanyl groups and control group（P0.05）.The productions of MMP-2 and MMP-9 proteins reduced concentration-dependently when treated with fentanyl.There was statistically significant difference between each experimental group and control group（P0.01）.Conclusion：Fentanyl can inhibit the expressions of MMP-2 and MMP-9 in MCF-7 cells,which might be one of the molecule mechanisms of its effectively inhibiting the invasion capacity of MCF-7 cells.

关 键 词：枸橼酸芬太尼 人乳腺癌细胞 基质金属蛋白酶类 

分 类 号：R979.1[医药卫生—药品]