HPLC同时测定苍耳子中6个化学成分的含量  

HPLC simultaneous determination of the content of 6 constituents in Fructus Xanthii

作　　者：朵睿[1,2] 刘玉红[1] 王明奎[3] 易进海[1] 

机构地区：[1]四川省中医药科学院,成都610041 [2]泸州医学院,泸州646000 [3]中国科学院成都生物所,成都610041

出　　处：《药物分析杂志》2013年第1期78-82,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家重点基础研究发展计划(973计划)课题(2009CB522804)

摘　　要：目的:用HPLC同时测定苍耳子中新绿原酸、绿原酸、隐绿原酸、噻嗪双酮苷、1,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸6个化学成分的含量。方法:采用Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸为流动相进行梯度洗脱,流速1 mL·min-1,检测波长254 nm,柱温35℃。结果:新绿原酸、绿原酸、隐绿原酸、噻嗪双酮苷、1,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸色谱峰分离良好。进样量分别在0.012～1.2、0.028～2.8、0.012～1.2、0.014～1.4、0.024～2.4、0.011～1.1μg范围内线性关系良好。平均回收率(n=6)分别为102.3%、96.4%、99.1%、103.1%、103.0%、103.4%,RSD分别为1.8%、1.1%、1.8%、1.5%、2.0%、1.9%。结论:建立的苍耳子HPLC含量测定方法可用于苍耳子的质量控制。Objective：To establish a method for simultaneous determination of 6 constituents （neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, xanthiside, 1,5 -dieaffeoylquinic acid, and 4,5 -dicaffeoylquinic acid） in Fructus Xanthii. Methods：The assay was conducted on a Eclipse XDB -Cls column（250 mm ×4. 6 mm, 5μm） with methanol -0. 1% Ha PO4 as mobile phase. The flow rate was 1.0 mL.min- l, the detection wavelength was 254 nm and the column temperature was 35 ℃. Results：The correlation coefficient between concentration and chromatograph- ic peak area of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, xanthiside, 1,5 - dicaffeoylquinie acid, and 4,5 - dicaffeoylquinic acid was over 0. 9999 in the range of 0. 012 - 1.2,0. 028 - 2. 8,0. 012 - 1.2,0. 014 - 1.4,0. 024 - 2. 4,0. 011 - 1.1 μg, respectively. The average recoveries （ n = 6） of 6 constituents were 102. 3%, 96. 4% ,99. 1%, 103. 1%, 103.0%, and 103.4%, respectively ; RSDs were 1.8%, 1.1%, 1.8%, 1.5%, 2. 0%, and 1.9%, respectively. Conclusion： The established method can be used for the quality control of Fructus Xanthii.

关 键 词：中药质量控制 苍耳子 新绿原酸 绿原酸 隐绿原酸 噻嗪双酮苷 1 5-二咖啡酰奎宁酸 4 5-二咖啡酰奎宁酸 高效 液相色谱 

分 类 号：R917[医药卫生—药物分析学]