HPLC同时测定口炎清颗粒中7个有机酸的含量  

HPLC simultaneous determination of seven organic acids in Kouyanqing granules

作　　者：张燕[1] 何兵[2] 杨世艳[3] 

机构地区：[1]泸州医学院附属医院手麻科,泸州646000 [2]泸州医学院药物研究中心,泸州646000 [3]泸州医学院附属中医医院超声影像科,泸州646000

出　　处：《药物分析杂志》2013年第1期108-111,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：泸州市2010年重点科技项目(泸市财企[2010]41号);泸州医学院2010年青年基金(泸医院[2010]108号)

摘　　要：目的:建立HPLC同时测定口炎清颗粒中7个有机酸(新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸及4,5-二咖啡酰奎宁酸)含量的方法。方法:采用AkzoNobel Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,柱温30℃,以乙腈-0.1%磷酸水溶液进行梯度洗脱,流速1.0 mL·min-1,检测波长326 nm。结果:7个成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3%,加样回收率为97.75%～99.33%。结论:该方法可用于口炎清颗粒多指标质量控制。Objective：To establish an HPLC method for the simultaneous determination of 7 organic acids（neochlo- rogenic acid, chlorogenie acid, eryptochlorogenic acid, caffeic acid, 3,4 - dicaffeoylquinic acid, 3,5 - dicaffeoylquin- ic acid and 4,5 - dieaffeoylquinic acid） in Kouyanqing granules. Methods：The chromatographic separation was per- formed on a AkzoNobel Kromasil C18 （250 mm × 4.6 mm,5μm）column, the column temperature was maintained at 30 ℃. A gradient elution of acetonitrile -0. 1% phosphoric acid aqueous solution was adopted at the flow rate of 1 mL . min-1. The UV detection wavelength was 326 nm. Results：All the 7 compounds showed good linearity in the range of the test concentration. The RSDs of the precision, reproducibility and stability tests were less than 3%, the average recoveries of them were in the range of 97.75% -99.33 %. Conclusion：The method can be applied for the multi - indictor quality control of Kouyanqing granules.

关 键 词：高效液相色谱 口炎清颗粒 新绿原酸 绿原酸 隐绿原酸 咖啡酸 二咖啡酰奎宁酸 质量控制 标准提高 中药制剂 

分 类 号：R917[医药卫生—药物分析学]