RP-HPLC同时测定复方甘草片中4个成分的含量  

RP-HPLC simultaneous determination of four active components in compound liquorice tablets

作　　者：杨红娟[1] 郭伟斌[1] 郑淑凤[1] 胡猛慎[1] 黄剑英[1] 

机构地区：[1]厦门市药品检验所,厦门361012

出　　处：《药物分析杂志》2013年第1期141-145,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用高效液相色谱法同时测定复方甘草片中吗啡、磷酸可待因、甘草苷和甘草酸的含量。方法:采用PhenomenexLuna-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.02 mol·L-1磷酸二氢钾溶液(磷酸调pH至4.0)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长为220 nm(0～23 min,检测吗啡和可待因)、254 nm(23～50 min,检测甘草苷和甘草酸),柱温为35℃。结果:4个成分的峰面积与浓度的线性关系良好(r>0.9999);加样回收率为98.0%～100.8%。结论:该方法可用于复方甘草片中吗啡、磷酸可待因、甘草苷和甘草酸4个成分的含量测定。Objective：To develop an HPLC method for content determination of morphine, codeine phosphate, liquiritin, glycyrrhizic acid in compound liquorice tablets. Methods：The HPLC analysis was performed on a Phe- nomenex Luna C 18 （250mm×4.6mm,5μm） column. The mobile phase consisted of acetonitrile and 0.02 mol . L- 1 potassium dihydrogen phosphate （ pH was adjusted to 4. 0 by phosphoric acid） with a gradient elution at the flow rate of 1.0 mL . min -1. The detected wavelengths were set separately in different time period （0 - 23 min,220 nm for morphine and codeine ;23 -50min,254 nm for liquiritin and glycyrrhizic acid）, and the column temperature was 35 ℃. Results：Four active components were separated completely in 50 minutes, and had good linear relation- ship （r 〉 0. 9999 ） and recoveries（98.0% - 100. 8% ） in their respective detection scopes. Conclusion ：The method can be applied for simultaneous determination of four active components （ morphine, codeine phosphate, liquiritin, glycyrrhizic acid） in compound liquorice tablets.

关 键 词：复方甘草片 吗啡 磷酸可待因 甘草苷 甘草酸 高效液相色谱法含量测定 标准提高 

分 类 号：R917[医药卫生—药物分析学]