异氟醚预处理改善大鼠海马脑片缺氧损伤  

Improving hypoxic injury in rat hippocampal slices with isoflurane preconditioning

作　　者：韩传来[1] 王志萍[2] 江山[3] 

机构地区：[1]南京市中医院麻醉科,210001 [2]无锡市人民医院麻醉科 [3]江苏省麻醉医学研究所

出　　处：《临床麻醉学杂志》2013年第1期75-77,共3页Journal of Clinical Anesthesiology

摘　　要：目的以大鼠海马脑片为研究对象,采用微电极记录技术,观察异氟醚预处理对海马脑片缺氧损伤后顺向群锋电位(OPS)以及缺氧损伤电位(HIP)的影响。方法 SD大鼠60只,随机均分为五组:空白对照组(C组);实验对照组(EC组);异氟醚预处理组(IP组);iNOS阻断剂氨基胍(AG)组(AG组);异氟醚预处理+iNOS阻断剂组(IPAG组)。IP组给予3h异氟醚预处理,AG组给予iNOS阻断剂处理,IPAG组给予3h异氟醚预处理,同时给予iNOS阻断剂,取脑片后分别给予14min的缺氧处理,观察不同处理对海马脑片OPS恢复以及HIP的不同效应。结果 IP组的OPS的恢复程度、恢复率明显高于C组、EC组、AG组和IPAG组(P<0.05),IP组HIP持续时间明显长于C组、EC组、AG组和IPAG组(P<0.05),HIP的出现时间五组差异无统计学意义。结论异氟醚预处理可明显减轻海马脑片缺氧损伤;用iNOS阻断剂可去除这种保护作用,证明这种预处理作用与异氟醚预处理产生的iNOS有关。Objective Microelectrode recording technology was used on hippocampal slices to study the effect of preconditioning with isoflurane on orthodromic population spikes （OPS） and hypoxic injury potential （HIP） during and after hypoxia. Methods Sixty SD rats were randomly divided into control group （group C）, experiment control group （group EC）, isoflurane preconditioning group（group IP）,iNOS inhibitor group（group AG） and iNOS inhibitor ＋ Isoflurane preconditioning group（group IPAG）. Both group IP and IPAG was given 3 hours isoflurane inhaling preconditioning. A minoguanidine was intraperitoneally injected in group IPAG and group AG. The hippocampal slices were exposed to 14 minutes hypoxia. Then, the distinct reactions of hippocampal slices OPS and HIP was observed. Results The OPS recovery degree of group IP were significantly better than other groups（P〈0.05） ; The duration of HIP in the IP group was much longer than that in other groups （P〈0.05）. But onset time of HIP produced no statistically difference between each group. Conclusion Preconditioning with isoflurane can attenuate the injury of rat hippocampal slice during and＇ after hypoxia, which could be blocked by iNOS inhibitors. Thus, iNOS might play important effects in the protective effect of isoflurane.

关 键 词：异氟醚 预处理 海马脑片 缺氧 

分 类 号：R614[医药卫生—麻醉学]