脱氧核糖核酸电致化学发光传感技术的研究  

Research on DNA Electrochemiluminescence Biosensing

作　　者：张帆[1] 陈红[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200241

出　　处：《分析化学》2013年第1期1-9,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21075042)资助项目

摘　　要：电致化学发光(ECL)由电化学反应直接或间接引发,具有灵敏度高、选择性好、易实现实时化和集成化、可进行原位检测的优点,为生命科学进入到分子水平研究提供了有力的方法。脱氧核糖核酸(DNA)研究是生命科学领域中极为重要的内容,特定DNA序列的定量检测在法医鉴定、疾病预防、环境监测等领域,尤其是对疾病的早期准确诊断及治疗具有重要意义。DNA-ECL传感技术,被认为是最有发展前景的一类DNA分析方法,是当今生物学和医学领域的前沿性研究课题。本文重点评述了国内近5年DNA-ECL传感技术的研究工作,最后对其研究前景进行了展望。Electrochemiluminescence （ECL） is directly or indirectly caused by the electrochemical reactions. Due to its high sensitivity, specificity, easy realization of integration, real-time and in-situ detection, ECL provides powerful research tools and methods for the life sciences entering into the molecular level. DNA research is an extremely important aspect in life science and shows the significance in the areas of forensic identification, epidemic prevention and environmental monitoring, especially for the early diagnosis and disease treatment. DNA-ECL biosensing is considered as the most promising technique for DNA analysis and has been a hot research topic in the field of biology and medicine. This review summarizes the research of our country on DNA-ECL biosensing technologies in the recent five years.

关 键 词：电致化学发光 脱氧核糖核酸 信号扩增 评述 

分 类 号：O629.74[理学—有机化学]