2型糖尿病大鼠周围神经病变模型的建立及相关指标检测  

Establishment of type 2 diabetes model in rat with peripheral neuropathy and detection of indicators

作　　者：殷娟[1] 张明红[2] 杨红英[3] 

机构地区：[1]攀枝花市中心医院检验科,四川攀枝花617067 [2]攀枝花市仁和区人民医院检验科,四川攀枝花617061 [3]昆明医科大学第二附属医院检验科,云南昆明650001

出　　处：《国际检验医学杂志》2013年第1期1-2,5,共3页International Journal of Laboratory Medicine

摘　　要：目的建立2型糖尿病(T2DM)大鼠糖尿病周围神经病变模型,观察坐骨神经形态和功能变化。方法雄性SD大鼠44只,分为普通饲料对照(NC)组、高脂饲料对照(HC)组和糖尿病(DM)组。高脂饲料喂养加小剂量链脲佐菌素(STZ)注射诱导2型糖尿病8周后神经电生理检测坐骨神经动作电位传导速度,电镜检测坐骨神经形态学变化。结果诱导糖尿病8周后,部分大鼠出现神经病变,坐骨神经动作电位传导速度显著降低(P<0.05),并出现明显的脱髓鞘等病理变化。结论高脂饮食结合小剂量STZ注射可复制发病过程近似人类T2DM、伴周围神经病变的大鼠模型,可用于相关病理研究。Objective To establish a rat model featured peripheral neuropathy associated with type 2 diabetes mellitus（T2DM）.Methods 44 male SD rats were divided into normal diet control（NC） group,high fat diet control（HC） group and diabetes mellitus（DM） group.High fat diet plus small dose streptozotocin（STZ） were used to induce T2DM.8 weeks after inducing,nerve conduction velocity was detected by neuroelectrophysiology testing and morphology changes in sciatic nerve were detected by electron microscopy.Results 8 weeks after inducing,part of rats were coincidence with diabetic neuropathy,with lower sciatic nerve conduction velocity（P0.05）,and with apparent pathological changes such as demyelination.Conclusion High fat diet combined with small dose STZ could replicate the rat model with progress similar to T2DM of human.The model could be conducive to causing peripheral neuropathy,and could be used to study the pathology mechanisms.

关 键 词：糖尿病 2型 周围神经 神经电生理 显微镜检查 电子 大鼠 

分 类 号：R587.2[医药卫生—内分泌]