SD大鼠肾衰竭模型BMP-7和TGF-β_1表达水平分析及药物干预效应研究  

Expression of BMP-7 and TGF-1 in renal tissues of SD rat model of renal failure and the efficacy of different drugs

作　　者：解冰[1] 江利萍[1] 

机构地区：[1]镇江市中西医结合肾脏病研究所,江苏镇江212028

出　　处：《国际检验医学杂志》2013年第1期3-5,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨慢性肾衰竭肾组织骨形成蛋白-7(BMP-7)和转化生长因子β1(TGF-β1)表达水平及药物干预的作用。方法 SD大鼠5/6肾切除法构建慢性肾衰竭模型,荧光定量聚合酶链反应测定肾组织TGF-β1及BMP-7表达,观察各研究组肾组织TGF-β1及BMP-7表达情况及血尿素氮(BUN)、肌酐(Cr)及尿蛋白水平。结果与对照组比较,未处理组TGF-β1水平增高、BMP-7水平降低(P<0.05)。洛汀新组TGF-β1、BMP-7水平低于对照组(P<0.05),且BMP-7水平低于未处理组。肾衰康组TGF-β1水平低于病理组(P<0.05),BMP-7水平高于未处理组(P<0.05);洛汀新组及肾衰康组血BUN、Cr及尿蛋白水平均低于未处理组(P<0.05)。结论慢性肾衰竭肾组织TGF-β1表达增强、BMP-7表达降低;洛汀新可抑制TGF-β1表达,但无增强BMP-7表达的作用;肾衰康既能抑制TGF-β1表达,也能增强BMP-7表达。Objective To evaluate the expression of bone morphogenetic protein-7（BMP-7） and transforming growth factor-β1（TGF-β1） in renal tissues of chronic renal failure and the efficacy of drug intervention.Methods Chronic renal failure models were established by nephrectomize 5/6 kidney of SD rats,and the expression of BMP-7 and TGF-β1 of kidney were detected by fluorescence quantitative polymerase chain reaction,and the concentration of blood urea nitrogen（BUN）,creatinine（Cr） and urine protein of different groups were observed.Results The expression of TGF-β1 was significantly increased and BMP-7 decreased in untreated group compared with control groups（P0.05）.The expression of TGF-β1 was significantly decreased in Lotensin group,compared with control group（P0.05）,but the expression of BMP-7 was decreased,compared with control and untreated groups（P0.05）.The expression of TGF-β1 was significantly decreased in Lotensin group,compared with untreated group,otherwise,the expression of BMP-7 was opposite（P0.05）.The concentration of BUN,Cr and urine protein were significantly decreased in Lotensin group and Shenshuaikang group,compared with untreated group（P0.05）.Conclusion The expression of TGF-β1 might be increased and BMP-7 decreased in chronic renal failure.Lotensin could inhibit the expression of TGF-β1,but could not enhance the expression of BMP-7.Shenshuaikang could inhibit the expression of TGF-β1 and enhancing the expression of BMP-7.

关 键 词：骨形成蛋白-7 转化生长因子Β1 肾功能衰竭 慢性 洛汀新 肾衰康 

分 类 号：R692.5[医药卫生—泌尿科学]