慢性乙型肝炎患者HBsAg和HBsAb共阳性与preS区及S区突变关系的研究  

Correlation between mutations in preS/S gene region and coexistence of HBsAg and HBsAb in chronic hepatitis B patients

作　　者：邬兰[1] 杜同信[1] 焦杰[1] 王自正[1] 瞿卫[1] 颜宁[2] 俞杨[1] 

机构地区：[1]南京医科大学附属南京医院/南京市第一医院核医学科实验诊断部分子诊断室,江苏南京210006 [2]南京医科大学附属南京医院/南京市第一医院感染性疾病科,江苏南京210006

出　　处：《国际检验医学杂志》2013年第1期16-19,共4页International Journal of Laboratory Medicine

基　　金：南京市医学科技发展资金(卫生青年人才培养项目)资助项目(NJH201132)

摘　　要：目的探讨HBsAg、HBsAb共阳性慢性乙型肝炎患者HBVpreS及S基因突变的关系。方法对6例HBsAg、HBsAb共阳性慢性乙型肝炎患者(试验组)、9例HBsAg阳性、HBsAb阴性慢性乙型肝炎患者(对照组)进行HBVDNA的提取及HBV基因组preS和S基因的PCR扩增和测序,比较组间突变率差异。结果试验组与对照组所感染HBV均为B型或C型。试验组未检出preS区缺失突变,对照组检出1例preS1区15bp缺失。试验组与对照组比较,preS/S、preS、preS1、preS2区核酸点突变率无统计学差异(P>0.05),S区核酸点突变率有统计学差异(P<0.05)。在氨基酸水平上,试验组与对照组preS/S、preS、S、preS1、preS2区及α决定簇点突变率比较无统计学差异(P>0.05)。结论 HBVpreS基因的缺失及S基因的氨基酸突变均不是B型或C型慢性乙型肝炎患者出现HBsAg、HBsAb共阳性的决定性因素。Objective To explore the correlation between HBV preS/S gene and coexistence of HBsAg and HBsAb in patients with chronic hepatitis B. Methods HBV DNA,extracted from 6 cases of chronic hepatitis B patients positive with HBsAg and HI3 sAb（experimental group） and 9 cases of chronic hepatitis B patients positive with HBsAg and negative with HBsAb（control group） was detected for the mutation in preS and S gene. The mutation rate was compared between the two groups. Results Both the ex-perimental group and the control group were of HBV genotype B or C. There was no preS deletion in experimental group, while one case in control group showed 15 bp deletion in preS1 region. There was no statistical difference of nucleic acid point mutation rates in PreS/S,preS,preS1 and preS2 gene between the two groups（P〉0.05）, hut that in S gene was with statistical difference（P〈0.05）. The was no statistical difference of amino acid point mutation rates in preS/S, preS, S, preS1, preS2 gene and a determinant （aa124-147）（P〉0.05）. Conclusion Concurrent HBsAg and HBsAb might not be associated with HBV preS deletion or the mu tation of amino acids in S gene in patients with chronic HBV infection（genotype B or C）.

关 键 词：肝炎病毒 乙型 HBV S基因 HBV preS基因 基因缺失 

分 类 号：R512.62[医药卫生—内科学]