产妇外周血和胎儿脐血白细胞介素28启动子甲基化差异研究  

Study of the methylation difference in the promoter of interleukin 28 between maternal peripheral blood and fetal cord blood

作　　者：马建鸿[1] 张元珍[1] 祝成亮[2] 周春[1] 王燕[1] 陈红[1] 李家福[1] 

机构地区：[1]武汉大学中南医院妇产科,湖北武汉430071 [2]武汉大学人民医院检验科,湖北武汉430060

出　　处：《国际检验医学杂志》2013年第2期132-133,135,共3页International Journal of Laboratory Medicine

基　　金：湖北省科技攻关项目(2007AA301B37-2);武汉市科技攻关项目(200760423158)

摘　　要：目的探讨产妇外周血和胎儿脐血白细胞介素28(IL-28)启动子甲基化差异及其对IL-28表达的影响。方法采用甲基化特异性PCR(MSP)方法检测产妇外周血和胎儿脐血IL-28启动子甲基化水平的差异;分离外周血单核细胞(PBMC),比较DNA甲基化酶活性的差异;RT-PCR检测两者PBMC中IL-28mRNA的含量;采用ELISA法检测产妇外周血和胎儿脐血IL-28水平的差异。结果 IL-28启动子在胎儿脐血中均以甲基化形式存在,而在产妇外周血中为非甲基化形式;胎儿脐血PBMC细胞核甲基化酶活性升高(P<0.05);IL-28mRNA在产妇外周血PBMC中的表达水平高于胎儿脐血PBMC;胎儿脐血IL-28含量较产妇外周血低(P<0.01)。结论胎儿脐血细胞核内甲基化酶活性升高导致IL-28启动子高甲基化,进而引起IL-28的合成和分泌降低。Objective To explore the difference of interleukin 28（IL-28） promoter methylation between maternal peripheral blood and fetal cord blood and its effect on the expression of IL-28.Methods The methylation specific PCR（MSP）method was applied to detect the methylation of IL-28.The peripheral blood mononuclear cell（PBMC） was seperated from maternal peripheral blood and fetal cord blood in order to compare the differences in the DNA methyltransferase activity.The levels of IL-28 mRNA in PBMC were measured by RT-PCR,and changes of IL-28 were analyzed by ELISA.Results IL-28 promoter was in the form of methylation in fetal cord blood.The DNA methyltransferase activity was increased in PBMC of fetal cord blood（P0.05）.The expression levels of IL-28 mRNA was higher in PBMC of maternal peripheral blood,and IL-28 was reduced in fetal cord blood（P0.01）.Conclusion The increasing DNA methyltransferase activity in fetal cord blood could lead to high methylation of IL-28 promoter,which would cause the reduction of IL-28.

关 键 词：胎血 白细胞介素28 甲基化 

分 类 号：R714.5[医药卫生—妇产科学]