血培养病原菌分布及耐药性分析  

Distribution and drug resistance of pathogenic bacteria in blood culture

作　　者：刘行超[1] 莫姗[1] 高云[1] 曾桂芬[1] 费燕楠[1] 

机构地区：[1]中国人民解放军一八一医院检验科,广西桂林541002

出　　处：《国际检验医学杂志》2013年第2期140-142,共3页International Journal of Laboratory Medicine

摘　　要：目的了解该院血培养标本中病原菌的分布和耐药性,为临床合理选择抗菌药物提供依据。方法采用BacT/A-lert3D血培养仪进行血培养,ATB-expression微生物鉴定系统对细菌及真菌进行鉴定,K-B纸片法进行药敏试验,并进行统计学分析。结果 2495例血培养标本中检出阳性标本199例,阳性率为7.98%,其中革兰阴性菌96株,占48.24%;革兰阳性菌85株,占42.71%;真菌18株,占9.05%。大肠埃希菌和肺炎克雷伯菌对亚胺培南、美罗培南的敏感率为100.00%。金黄色葡萄球菌和表皮葡萄球菌对万古霉素、利奈唑胺的敏感率为100.00%。结论临床医生应高度重视血培养结果,根据药敏试验结果合理使用抗菌药物,防止耐药菌株的产生。Objective To investigate the distribution and the drug resistance of pathogenic bacteria in blood culture of the hospital,in order to provide evidence for rational selection of antimicrobial agents.Methods Blood samples were cultivated with Bact/Alert 3D system,pathogenic bacteria were identified with ATB-expression system.Drug sensitivity tests were carried out by K-B method.The test results were analyzed with WHONET5.4 software.Results Among 2 495 blood culture samples,199 samples were positive and the positive rate was 7.98%.There were 96 strains of Gram-negative bacteria（48.24%）,85 strains of Gram-positive bacteria（42.71%） and 18 strains of fungi（9.05%）.Escherichia coli and Klebsiella pneumoniae had 100.00% sensitivity to imipenem and meropenem.Staphylococcus aureus and Staphylococcus epidermidis had 100.00% sensitivity to vancomycin and linezolid.Conclusion Clinical doctors should pay great attention to the results of blood culture and rationally use antimicrobial agents according to drug resistance test,so as to prevent and control drug-resistant bacteria.

关 键 词：血培养 病原菌 抗药性 

分 类 号：R446.5[医药卫生—诊断学]