用Ames试验检测水源水和自来水中的遗传毒性  

Determination of Genetic Toxicity of Source Water and Drinking Water by AMES Test

作　　者：方东[1] 梅卓华[1] 楼霄[1] 章弼 

机构地区：[1]南京市环境监测中心站,江苏南京210013

出　　处：《环境监测管理与技术》2000年第1期20-22,共3页The Administration and Technique of Environmental Monitoring

摘　　要：用鼠伤寒沙门氏菌/哺乳动物微粒体酶系的Ames试验,研究了不同季节的水源水及管网自来水中的遗传毒性。以XAD2树脂为吸附剂,以丙酮甲醇的混合液为洗脱液,浓缩水样中的有机物,并对部分阳性水样进行有机成分的定性分析。结果发现:13个水样中有7个样品在不需要代谢活化系统S9的情况下,可诱导鼠伤寒沙门氏菌碱基移码型菌株的回复突变;不同水样在不同季节有不同的诱导作用;同时通过GC/MS方法分析,发现阳性水样中存在有多种美国EPA所列的129种优先污染物及其他属于黑名单上的有毒有害物质,表明水源水与自来水的水质已受到污染。Genetic toxicity of source water and drinking water in different seasons was researched by Ames test with the application of Salmonella/microsonal enzyme of mammal.After the adsorption of organics with XAD 2 as adsorbant and acetone methanol as desorbant,organics in positive samples were identified.The results showed that 7 of 13 samples led reversion mutation of base shift mutagenisis without metabolic activation S 9, and different samples from different seasons had different leading function. GC/MS analysis showed that positive samples had pollutants in US EPA 129 priority pollutants or other lists of priority pollutants, which meant the water was polluted.

关 键 词：AMES试验 GC\MS 水源水 自来水 遗传毒性 

分 类 号：X832.02[环境科学与工程—环境工程] TU991.21[建筑科学—市政工程]