甘油提高巴氏芽孢杆菌脲酶的热稳定性  

Study on enhancing urease thermostability of Bacillus pasteurii by glycerol

作　　者：许燕波[1,2] 钱春香[1,2] 陆兆文[1,2] 

机构地区：[1]东南大学材料科学与工程学院,南京211189 [2]东南大学江苏省土木工程材料重点实验室,南京211189

出　　处：《东南大学学报（自然科学版）》2013年第1期147-151,共5页Journal of Southeast University：Natural Science Edition

基　　金：江苏省科技支撑计划资助项目(BK2010062)

摘　　要：研究了保存时间和保存温度对碳酸盐矿化菌脲酶活性的影响,发现在低温4℃条件下,脲酶活性在29 d内出现不同程度下降;常温短期储存时,酶活性可以保持3 d,3 d后酶活性值下降明显.为提高脲酶的热稳定性,向碳酸盐矿化菌中添加甘油作为保护剂,常温下添加总体积分数20%的甘油,可以提高酶的稳定性,脲酶活性在30℃下3 d内保持不变,但甘油保护作用有限,高温储存超过7 d后酶活性值下降明显;而在保存温度4℃条件下,添加体积分数为10%的甘油,碳酸盐矿化菌可以储存30 d,底物分解量可以达到初始菌液的95%.Effect of preservation time and temperature on the carbonate-mineralization microbe urease activity is studied. The urease activity decreases in 29 days at 4℃ while it can be kept for 3 days at normal temperature before obvious decrease afterwards. Glycerol is selected as the protective additive to enhance the urease thermostability. The urease activity keep stable for 3 days at 30 ℃, and the best volume fraction for glycerol is 20% at normal temperature. However its effect of preservation is limited, and the urease activity decreases obviously after 7 days at high store temperature. The decomposition ability can reach up to 95% at 4 ℃ after 30 days with 10% glycerol, and the carbonate-mineralization microbe can be kept in reserve for a month.

关 键 词：碳酸盐矿化菌 脲酶 甘油 热稳定性 

分 类 号：Q89[生物学]