人类巨细胞病毒感染对血管平滑肌细胞脂代谢相关基因表达的影响  

The Influence of HCMV Infection on Expression of Genes Associated With Lipid Metabolism in Vascular Smooth Muscle Cells

作　　者：陈华[1,2] 刘伟[1,2] 程洁 徐玉芬[1,2] 李玲芳[3] 邓桢翰[3] 陈利玉[4] 刘水平[4] 邬国军[4] 

机构地区：[1]中南大学湘雅医学院长沙三临床学院 [2]长沙市第三医院检验科,湖南长沙410015 [3]中南大学湘雅医学院 [4]中南大学基础医学院微生物学系

出　　处：《中南医学科学杂志》2013年第1期17-20,共4页Medical Science Journal of Central South China

基　　金：湖南省卫生厅科研基金(B2008-079);湖南省自然科学基金重点项目(10JJ2012)

摘　　要：目的研究人类巨细胞病毒感染对体外培养血管平滑肌细胞脂代谢相关基因表达的影响,探讨该病毒致动脉粥样硬化(As)的机制。方法以1 MOI人类巨细胞病毒感染体外培养的血管平滑肌细胞,利用基因表达谱芯片技术检测细胞脂代谢相关基因的表达变化。结果人类巨细胞病毒感染血管平滑肌细胞后,细胞内与脂代谢有关的基因表达出现明显的异常,其中表达上调的基因主要有低密度脂蛋白受体相关蛋白10、11、12、极低密度脂蛋白受体、B型清道夫受体、HMG-CoA合成酶、HMG-CoA还原酶、酰基辅酶A∶胆固醇酰基转移酶及脂肪酶合成酶等基因;表达下调的主要有载脂蛋白A1、载脂蛋白M和载脂蛋白H等基因。结论人类巨细胞病毒感染可能通过改变血管平滑肌细胞脂代谢相关基因的表达而参与As的发病过程。Objective To investigate the effect of human cytomegalovirus infection on expression of genes associated with lipid metabolism in vascular smooth muscle ceils cultured in vitro, and to clarify the possible mechanisms of atherosclero- sis induced by this virus. Methods The expression change of genes associated with lipid metabolism in vascular smooth muscle cells cultivated in vitro after human cytomegalovirus infection were scanned by gene expression profiling chip. Re- sults There was an obvious abnorm~ expression profile of genes associated with lipid metabolism in vascular smooth muscle cells after challenged with human cytomegalovirus, the main up-regulated genes were low density lipoprotein receptor-related protein,very low density lipoprotein receptor, scavenger receptor class B, HMG-CoA synthetase, HMG-CoA reductases, acyl- Coenzyme A：cholesterol acyltransferase,fatty acid synthase,and so on,while the main down-regulated genes were apolipopro- rein A-I （APOA1）, apolipoprotein M （APOM） and apolipoprotein H （APOH）. Conclusion The involvement of human cytomegalovirus in the pathogenesis of atherosclerosis may be by changing the expression level of lipid metabolism genes.

关 键 词：动脉粥样硬化 人类巨细胞病毒 脂代谢 基因表达谱芯片 血管平滑肌细胞 

分 类 号：R37[医药卫生—病原生物学]