进境菠菜种子中藜草花叶病毒的免疫捕获RT-PCR检测  

Immuno capture RT-PCR for the detection of Sowbane mosaic virus from imported spinach seeds

作　　者：冯黎霞[1] 何瑞芳[1] 钟国强[1] 张洪玲 胡学难[1] 

机构地区：[1]广东检验检疫技术中心,广东广州510623 [2]番禺出入境检验检疫局

出　　处：《植物检疫》2013年第1期33-35,共3页Plant Quarantine

基　　金：国家国际科技合作计划资助(2011DFB30040)

摘　　要：本研究基于藜草花叶病毒(Sowbane mosaic virus,SoMV)外壳蛋白基因的保守区域设计特异性引物,建立了SoMV的免疫捕获反转录PCR(IC-RT-PCR)方法。应用建立的IC-RT-PCR方法,从来自丹麦和荷兰的进境菠菜种子中检测到SoMV。这是我国进境种子中截获SoMV的首次报道。This research had analyzed different isolates of SoMV coat protein nucleotide sequences,and designed a pair of specific primer to detect SoMV by one step immuno capture RT-PCR.Using this IC-RT-PCR method,we respectively have detected SoMV on the imported spinach（Spinacia oleracea） seeds from Denmark and Netherlands.This is the first report of the interception of SoMV from imported seeds.

关 键 词：藜草花叶病毒 IC-RT-PCR 菠菜 检测 

分 类 号：S41-30[农业科学—植物保护]