冬生疫霉的实时荧光PCR检测方法  

Detection of Phytophthora hibernalis by real-time fluorescent PCR

作　　者：杜洪忠[1] 吴品珊[1] 严进[1] 张秋娥[1] 

机构地区：[1]中国检验检疫科学研究院植物检疫研究所,北京100029

出　　处：《植物检疫》2013年第1期36-39,共4页Plant Quarantine

基　　金：中国检验检疫科学研究院基本科研业务费(2010JK005);质检公益性行业科研专项(201010256);"十二五"国家科技支撑计划(2012BAK11B02)

摘　　要：冬生疫霉(Phytophthora hibernalis)是我国检疫性病原菌。本研究根据冬生疫霉的ITS基因序列,设计了实时荧光PCR引物PH-F和PH-R及TaqMan-MGB探针PH-Pr,建立了冬生疫霉的实时荧光PCR检测方法,可检测到冬生疫霉DNA最低浓度为50 fg/μL,而冬生疫霉的近似种、近缘种和空白对照,无荧光信号增加。因此,利用该方法可以稳定、高效的检测出冬生疫霉。Phytophthora hibernalis is one of quarantine pathogen of China.One pair of real-time PCR primers PH-F,PH-R and Taqman-MGB probe PH-Pr for P.hibernalis were designed basing on the sequence of ITS genes,and a real time PCR method was developed.More than 50 fg/μL DNA of P.hibernalis could be detected.Other similar species of P.hibernalis and blank had none fluorescent signals.Therefore,P.hibernalis can be detected steadily and rapidly by using the real-time PCR.

关 键 词：PHYTOPHTHORA hibernalis 实时荧光PCR 检测 

分 类 号：S41-30[农业科学—植物保护]