2种常见伞滑刃线虫的特异PCR检测方法  

Special PCR detection of 2 common Bursaphelenchus spp.

作　　者：熊玉芬[1] 王旭[1] 葛建军[1] 顾建锋[2] 王金成[3] 郑春生 

机构地区：[1]中国检验检疫科学研究院,北京100029 [2]宁波出入境检验检疫局技术中心 [3]天津出入境检验检疫局技术中心 [4]北京检验检疫技术中心

出　　处：《植物检疫》2013年第1期49-53,共5页Plant Quarantine

基　　金：中国检验检疫科学研究院基本科研业务费(2011JK003);质检公益性科研专项(201010256);质检总局科研专项(2011IK156;2011IK183);"十二五"国家科技支撑计划(2012BAK11B03)

摘　　要：包括松材线虫和椰子红环腐线虫在内的伞滑刃属线虫形态上相似性高,形态鉴定难度较大。本文通过对伞滑刃属常见线虫群体ITS2区序列比对、分析,设计了木质包装材料检疫中常见的泰国伞滑刃线虫和豆伞滑刃线虫特异引物对各1对,并研究了这2种常见伞滑刃线虫的PCR检测方法,使用本文特异引物可分别特异、快速检测出这两种线虫。It＇s relatively difficult to distinguish species from Bursaphelenchus nematodes,including B.xylophilus and B.cocophilus which were important plant quarantine nematodes,due to their high morphology similarity.Two PCR（Polymerase Chain Reaction） special primer pairs for B.thailandae and B.doui which were frequently seen in wood packages,were designed individually,through alignment and anylysis of internal transcribed spacer 2（ITS2） regions of Bursaphelenchus nematodes.Using the two primer pairs,special and rapid methods for identifying B.thailandae and B.doui were complished.

关 键 词：泰国伞滑刃线虫 豆伞滑刃线虫 PCR检测 

分 类 号：S41-30[农业科学—植物保护]