棉花皱叶病毒分子检测方法  

Molecular detection of Cotton Leaf Crumple Virus

作　　者：雷荣[1] 辛言言[1] 乔文婕[1] 周雪平[2] 鲁洁[1] 马洁[1] 张永江[1] 

机构地区：[1]中国检验检疫科学研究院,北京100029 [2]浙江大学

出　　处：《植物检疫》2013年第1期53-57,共5页Plant Quarantine

基　　金：质检公益性行业科研专项(201010256;201110035;201210214)

摘　　要：棉花皱叶病毒(Cotton leaf crumple virus,CLCrV)是典型的双生病毒,含A和B两个组分。根据B组分的保守序列设计的一对普通PCR引物,得到约为636 bp的片段,实验结果显示能特异性地扩增CLCrV B组分,棉花曲叶病毒(Cotton leaf curl virus,CLCuV)、非洲木薯花叶病毒(African cassava mosaec vi-rus,ACMV)、花椰菜花叶病毒(Cauliflower mosaic virus,CaMV)及番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)不干扰普通RCR方法对CLCrV的检测。设计的实时荧光PCR引物和探针也能特异性地检测棉花皱叶病毒,而CLCuV、ACMV和TYLCV不影响实时荧光PCR对CLCrV的检测。Cotton leaf crumple virus（CLCrV）is a whitefly-transmitted begomovirus,whose genome is segmented into two segments（referred to as DNA A and DNA B）.One pair of PCR primers were designed according to conserved regions of published sequences in GenBank.Electrophoretic analysis of the PCR products indicated that the amplified fragment of CLCrV DNA-B was approximately 636 bp,which is specific for the PCR of CLCrV.The designed real-time PCR primers and probe can also detect CLCrV DNA B with good specificity and high sensitivity.

关 键 词：棉花皱叶病毒 PCR 实时荧光PCR 检测 

分 类 号：S41-30[农业科学—植物保护]