水稻Xa7基因抗白叶枯病应答中的活性氧代谢分析  

Metabolism of reactive oxygen species in resistant response of rice Xa7 gene to bacterial blight

作　　者：马伯军[1] 郑茜茜[1] 张萍华[1] 胡娜娜[1] 陈析丰[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2013年第1期1-5,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家重大科技专项(2011ZX08009-003-001);国家自然科学基金资助项目(31171519;31101130);浙江省自然科学基金资助项目(Y3110234;Y3100531);浙江省重点科技创新团队项目(2010R50024);浙江省公益性技术应用研究计划项目(2011C22005)

摘　　要：白叶枯病是由革兰氏阴性黄单孢菌水稻变种(Xanthomonas oryzae pv.Oryzae,Xoo)所引起的一种世界性水稻细菌病害.水稻Xa7基因是一个具有广谱抗性的显性抗白叶枯病基因.通过对水稻抗病品种IRBB7(含Xa7)和感病对照IR24接种白叶枯菌PXO86,发现:在叶片的病原菌侵染部位,IRBB7比IR24的活性氧(H2O2和O2-)积累更快且含量更高;与活性氧代谢相关的酶,如超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和过氧化物酶的活性也更高.推测活性氧的代谢调节可能在Xa7基因介导的抗病反应中起作用.Bacterial blight （ BB ） , caused by Xanthomonas oryzae pv. Oryzae, is one of the most serious worldwide disease of rice. Xa7 is a broadly dominant resistance gene directed against BB. The resistant rice cultivar IRBB7 （ with Xa7 ） and susceptible control IR24 were inoculated with strain PXO86 of BB. Comparison to the IR24, the leaves of IRBB7 accumulated reactive oxygen species （ROS; H2O2 and 02 - ） faster and higher, the activity of active oxygen metabolism-related enzymes （ SOD, CAT, APX and POD ） of IRBB7 was higher in the infect site. It was suggested that the regulation of ROS metabolism might be helpful for the Xa7- mediated disease resistance response.

关 键 词：水稻 抗白叶枯病 Xa7基因 活性氧 

分 类 号：Q943[生物学—植物学] S432.1[农业科学—植物病理学]