Hyphomicrobium sp.MAP-1菌株修复甲胺磷乙酰甲胺磷和水胺硫磷污染土壤的实验研究  

Bioremediation on the Soil Contaminated by Methamidophos,Acephate and Isocarbophos by Hyphomicrobium sp.MAP-1

作　　者：王莉[1,2] 凌琪[1] 伍昌年[1] 李顺鹏[2] 蒋建东[2] 王敏[1] 

机构地区：[1]安徽建筑工业学院环境与能源工程系,合肥230022 [2]南京农业大学生命科学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《农业环境科学学报》2013年第1期81-87,共7页Journal of Agro-Environment Science

基　　金：国家自然科学基金(21107002);安徽建筑工业学院博士基金(2011);住房与城乡建设部科学技术项目计划(2009-K6-3);安徽省自然科学基金项目(11040606M99)

摘　　要：在实验室条件下,评估了菌株Hyphomicrobium sp.MAP-1对甲胺磷、乙酰甲胺磷和水胺硫磷污染土壤的修复能力。革兰氏阴性菌MAP-1在土壤中能很好的定殖,以1×107CFU·g-1soil的浓度接种于土壤,15d后菌株浓度仅下降了1个数量级。土壤非灭菌状态下,30℃pH6.8,接种量为1×107CFU·g-1soil时,9d内可分别将土壤100mg·kg-1甲胺磷、100mg·kg-1乙酰甲胺磷完全降解,13d内将50mg·kg-1水胺硫磷完全降解。添加甲醇和加大接种量能促进土壤中三种有机磷农药降解,土壤是否处于淹水状态对其降解影响不大。在20～30℃之间,土壤中三种农药有比较好的去除效果。土壤投加菌株MAP-1,第10d可使100mg·kg-1甲胺磷和100mg·kg-1乙酰甲胺磷抑制的土壤脱氢酶、蔗糖酶和脲酶活性恢复至施农药前水平,对50mg·kg-1水胺硫磷抑制的这三种土壤酶活性也有较大的恢复作用。可见将菌株MAP-1应用于降低甲胺磷、乙酰甲胺磷和水胺硫磷对土壤的潜在风险是可接受的。The ability of bioremediation on contamined soil by methamidophos,acephate,and isocarbophos for Hyphomicrobium sp.MAP-1 was assessed under laboratory conditions.It was found that the gram-stain-negative strain MAP-1 could well colonizated in soil,and concentration of MAP-1 only decreased by one order of magnitude after 15 days,when inculating MAP-1 into soil at the inculum size of 1×107 CFU·g-1 soil.Under these conditions：non-sterilization soil,30 ℃ pH 6.8,the inculum size of 1×107 CFU·g-1 soil,the strain MAP-1 was able to completely respectively degradate 100 mg·kg-1 methamidophos and 100 mg·kg-1 acephate within 9 days,completely remove 50 mg·kg-1 isocarbophos within 13 days.The addiation of methanol or increase of inoculum of strain MAP-1 in soil,could accelerate the degradation of three organophosphorus pesticides.Whether the soil was flooded or not,no notable effect on degradation was found.Between 20 ℃ and 30 ℃,the strain MAP-1 could efficiently remove three organophosphorus pesticides from soil.Adding the strain MAP-1 to soil,in the tenth day,the actitivity of dehydrogenase,invertase,and urease,inhibitted by 100 mg·kg-1 methamidophos or 100 mg·kg-1 acephate was recovered to the lever without pesticide,oppressed by 50 mg·kg-1 isocarbophos was also restored to a great extent.As a result,it was feasible for applying the strain MAP-1 to reduce the potential risks on soil by methamidophos,acephate,and isocarbophos.

关 键 词：Hyphomicrobium SP MAP-1 甲胺磷 乙酰甲胺磷 水胺硫磷 土壤 生物修复 

分 类 号：X53[环境科学与工程—环境工程]