广东四会邓村河水体中雌/雄激素物质的含量及分布  

Concentrations and Distribution of Estrogenic and Androgenic Chemicals in Water Collected from Dengcun River,Sihui City,Guangdong Province

作　　者：侯丽萍[1,2] 刘珊[1] 方展强[3] 应光国[1] 

机构地区：[1]中国科学院广州地球化学研究所,有机地球化学国家重点实验室,广州510640 [2]广州大学华南生物多样性保护与利用重点实验室,广州510006 [3]华南师范大学生命科学学院,广东省高等学校生态与环境科学重点实验室,广州510631

出　　处：《农业环境科学学报》2013年第1期135-140,共6页Journal of Agro-Environment Science

基　　金：国家自然科学基金(20977092);广东省教育厅育苗工程项目(2012LYM_0100);广州大学新苗计划

摘　　要：对受造纸废水污染的广东四会邓村河水体中存在的类雌/雄激素物质的数量和种类进行监测,以确定造成生活在邓村河水域的食蚊鱼发生性逆转的目标化合物。采用LC-MS/MS方法,于2011年夏季对邓村河设置包括对照点在内的4个检测点,对水体中的5种雌激素物质和10种雄激素物质的含量作了定量检测。结果显示,雌激素物质中雌二醇(17Α-estradiol,E2)、雌三醇(estriol,E3)、炔雌醇(17Β-ethinylestradiol,EE2)和已烯雌酚(diethylstilbestrol,DES)在各检测点均未检出,仅检出雌酮(estrone,E1)1.97～5.09ng·L-1;雄激素类物质中1,4雄烯二酮(androstadienedione,ADD)、17α-勃酮(17α-boldenone)、17β-勃酮(17β-boldenone)、雄烯二酮(androstenedione,AED)、睾酮(testosterone)、炔诺孕酮(norgestrel)、反式雄酮(epi-androsterone)、雄酮(androsterone)、孕酮(progesterone)、羟甲雄烷吡唑(stanozolol)均有检出,其含量分别为13.47～30.46、1.50～3.69、1.50～3.69、1.89～6.77、0.89～1.43、1.97～2.57、6.29～18.93、3.58～12.05、1.42～2.86、0.69～0.70ng·L-1。各采样点被检出的雌/雄激素物质含量均高于对照点(REF)的含量,其中E1在各个点都有检出,最高含量为5.29ng·L-1;采样点A的ADD、AED和Progesterone含量最高,分别为30.46、6.77、2.86ng·L-1。结果表明,邓村河水主要被E1、ADD、AED和Progesterone污染,在引起该水域生活的食蚊鱼雌/雄激素生物效应中起到重要作用。This paper monitored the content of estrogen and androgen in the polluted water samples from Dengcun River and intended to determine the target compound which caused the sex reversal of mosquitofish.The LC-MS/MS method was introduced along with the setting of four test points and one control point by Dengcun River in summer 2011.Five types of Estrogenic and ten types of Androgenic in each water samples were detected quantitatively by this method.The results showed that only the estrone（E1）was detected in estrogenic hormone,and the range of E1 was 1.97～5.29 ng·L-1.Androgenic hormone androstadienedione（ADD）,17α-boldenone,17β-boldenone,androstenedione（AED）,testosterone,norgestrel,epi-androsterone,androsterone,progesterone and stanozolol were detected,the range of content were 13.47～30.46,1.50～3.69,1.50～3.69,1.89～6.77,0.89～1.43,1.97～2.57,6.29～18.93,3.58～12.05,1.42～2.86 and 0.69～0.70 ng·L-1,respectively.The content of Estrogen and Androgen was found to be higher in each sampling points than that in the control point（REF）.E1 was detected in all test points and has a highest level of 5.29 ng·L-1.Sampling point A had the highest ADD,AED and progesterone content up to 30.46,6.77 and 2.86 ng·L-1,respectively.The results indicated Dengcun River was mainly polluted by E1,ADD ADE and Progesterone,which caused the biological effect of sex reversal of mosquitofish.

关 键 词：雌 雄激素物质 定量分析 邓村河 四会市 

分 类 号：X832[环境科学与工程—环境工程]