液-质联用法测定补虚颗粒中升麻素苷的含量  

LC-MS/MS determination of prim-O-glucosylcimifugin in Buxu granule

作　　者：陈琴华[1,2] 李鹏[1,2] 朱军[1,2] 

机构地区：[1]湖北医药学院附属东风医院药学部,湖北十堰442008 [2]湖北医药学院药物分析与筛选研究所,湖北十堰442008

出　　处：《中国医院药学杂志》2013年第2期101-103,共3页Chinese Journal of Hospital Pharmacy

基　　金：湖北省教育厅科研研究计划项目(编号:D20122401和B20122424)

摘　　要：目的:通过液相色谱-串联质谱(LC-MS/MS),采用选择监测离子法测定补虚颗粒中升麻素苷的含量。方法:样品用60%甲醇提取,通过液相色谱-串联质谱测定其中升麻素苷的含量,在正离子模式下,采用选择离子监测(SIM)方式进行测定,其监测离子对为m/z469→307。结果:升麻素苷在1～100μg.mL-1范围内线性关系良好(r=0.998 3),平均回收率96.0%,检测限为1.0ng.mL-1。结论:该方法快速、灵敏,结果准确,适用于复杂中药基质中升麻素苷的检测。OBJECTIVE To establish a rapid and sensitive method for the determination of prim-（Yglucosylcimifugin （POG） in Buxu granule by LC-MS/MS using selection ion monitoring （SIM）. METHODS The sample was extracted by 60% aqueous methanol. The mass spectrometer was operated in the positive ionization electrospray （ESI） mode using SIM. The lransitions m/z 469→3117 was used to quantify POG. RESULTS The method had satisfactory linearity in the range of 1 - 101） μg.mL-1 with a regression coefficient of （0. 998 3, the limit of detection （LOD） was 1.0 ng.mL-1 , and the mean recovery was 96%. CONCLUSION The method is sensitive, simple and accurate, and proved to be suitable in the determination of POG in the com- plicated traditional Chinese medicine matrices.

关 键 词：液相色谱-串联质谱 升麻素苷 补虚颗粒 

分 类 号：R927.2[医药卫生—药学]