3T3-L1前脂肪细胞分化过程中miRNAs基因的表达变化  

Expression Changes of miRNAs in the Differentiation of 3T3-L1 Pre-adipocytes

作　　者：张星星[1] 李杨[1] 周见至[1] 贾乙[1] 刘雅[1] 李晓辉[1] 

机构地区：[1]第三军医大学药学院药物研究所,重庆400038

出　　处：《中国药房》2013年第5期401-404,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81072630;30973523);重庆市自然科学基金资助项目(No.CSTC2010BB5169)

摘　　要：目的:探讨3T3-L1前脂肪细胞诱导分化过程中miRNAs基因的表达变化。方法:运用经典的"激素鸡尾酒"法建立3T3-L1前脂肪细胞分化模型。采用实时定量聚合酶链式反应方法检测不同时相点(0d、2h、2d、4d)细胞分化相关标志物的表达和20种miRNAs的表达变化,采用生物信息学方法对变化显著的miRNAs进行靶基因预测。结果:成功建立3T3-L1前脂肪细胞分化模型。不同时相点细胞分化相关标志物基因水平提示3T3-L1前脂肪细胞分化为脂肪细胞。20种miRNAs中以miR-126、miR-214、miR-320、miR-351的表达最为显著。靶基因预测结果显示,miR-126靶基因有影响细胞增殖的转录因子Crk、代谢相关基因Irs1等;miR-214靶基因有转录因子Zbtb20、Kpna1等;miR-320靶基因有影响代谢相关基因Igf2bp3、Gxylt1等;miR-351靶基因有Myt1、Mcl1、Bmf等。结论:miR-126、miR-214、miR-320、miR-351可通过影响基因转录及细胞代谢水平,从而对3T3-L1前脂肪细胞分化发挥作用,为开发治疗肥胖等疾病的新药提供了新的研究思路。OBJECTIVE： To explore the expression change of miRNAs in the differentiation of 3T3-L1 pre-adipocytes. METH- ODS： The model of 3T3-L1 pre-adipocytes differentiation was established by the classic ＂hormone cocktail＂ method. Changes of markers and 20 kinds of miRNAs were detected by Real-time PCR at different time points （0 d, 2 h, 2 d, 4 d）. Target genes of significantly changed miRNAs were predicted by bioinformatics. RESULTS： 3T3-L1 pre-adipocytes differentiation was established successfully. The expression of marks suggested 3T3-L1 pre-adipocytes differentiated into adipocytes. Among 20 kinds of miRNAs, the expressions of miR-126, miR-214, miR-320, miR-351 in these miRNAs were the most significant. The predicting results showed that the target genes of miR-126 included transcription factor Crk which affected cell proliferation, and Irsl related to me- tabolism, miR-214 target genes contained transcription factor Zbtb20, Kpnal; miR-320 target genes contained Igf2bp3 and Gxyltl which affected metabolism; miR-351 target genes included Mytl, Mcll, Bmf, etc. CONCLUSIONS： miR-126, miR-214, miR-320, miR-351 may play an important role on the differentiation of 3T3-L1 pre-adipocytes by affecting gene transcription and metabolic changes. It provides a new idea for drug research on obesity.

关 键 词：MIRNAS 3T3-L1 脂肪细胞 分化 

分 类 号：R965[医药卫生—药理学] R723.14[医药卫生—药学]