中国北方四省小麦丛矮病病原鉴定  

Molecular detection and identification of the pathogen causing wheat rosette stunt disease in the north of China

作　　者：段西飞[1] 邸垫平[1] 张爱红[1] 苗洪芹[1] 

机构地区：[1]河北省农林科学院植物保护研究所,保定071000

出　　处：《植物病理学报》2013年第1期91-94,共4页Acta Phytopathologica Sinica

基　　金：抗病虫转基因小麦新品种培育专项(2008ZX08002-001)

摘　　要：小麦丛矮病是由灰飞虱（Laodelphax striatellus Fallen）传播的一种病毒病，具有流行性和暴发性，可致小麦减产甚至绝收。小麦丛矮病的典型症状表现为叶片有黄绿相间条纹，分蘖增多，植株矮化，形成明显的丛矮状。冬前感病株大部分不能越冬而死亡，冬前未显症和早春及拔节后感病植株上部叶片显条纹，By biological identification and RT-PCR method it was found that Northern cereal mosaic virus （NCMV） was the pathogen of wheat rosette stunt disease. It was showed that 91.3% of the samples were posi- tive in 367 symptomatic wheat plants collected from Hebei, Henan, Shandong and Shanxi Provinces of China in 2010. The NCMV N, P, M, G and L gene sequences were amplified and obtained by RT-PCR with the primers designed according to the target gene. BLAST analysis revealed that the identity of nucleotide sequences derived from Hebei, Henan, Shandong and Shanxi isolates was 98.9%-99.3% and greater than 99% of deduced amino acid sequences. The identity of nucleotide and deduced amino acid sequences between these Chinese and Japanese NCMV isolates was 92.2%- 94% and 98.6%- 99.4%, respectively. Sequence analysis showed a high level of sequence conservation among NCMV isolates. However, there was a small variation between China and Japan isolates.

关 键 词：丛矮病 小麦 病原鉴定 中国北方 植株矮化 上部叶片 症状表现 灰飞虱 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]