来源于砂梨和桃的苹果褪绿叶斑病毒分离物部分CP基因和3'端非翻译区的序列分析  

Sequence analysis of partial CP gene and 3′-terminal UTR of Apple chlorotic leaf spot virus isolates from sand pear and peach

作　　者：王利平[1,2] 洪霓[1,2] 宋艳苏[1,2] 王俊珍[1,2] 马小方[1,2] 胡国君[1,2] 王国平[1,2] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]华中农业大学湖北省作物病害监测与安全控制重点实验室,武汉430070

出　　处：《植物病理学报》2013年第1期104-109,共6页Acta Phytopathologica Sinica

基　　金：教育部新教师基金(20100146120037);中央高校基本科研业务费专项资金资助(20100146120037);国家梨产业技术体系(nycytx-29-08)

摘　　要：苹果褪绿叶斑病毒（Apple chlorotic leaf spot virus，ACLSV）是引起果树病害的一种重要病毒。ACLSV寄主范围广、发生较普遍，可侵染苹果、梨等仁果类果树和桃、扁桃、李、樱桃、杏等核果类果树，据报道我国梨产区感染ACLSV达80％以上。The 3＇-terminal genome of 25 Apple chlorotic leaf spot virus （ACLSV） isolates from sand pear （Pyrus pyrifolia） in Wuhan and Hangzhou, and 2 isolates from peach in Wuhan and Xian were amplified by RT-PCR. The sizes of cloned fragments were 674, 679,680 and 686 bp, respectively, including partial coat protein （CP） gene and 3＇-terminal untranslated region （3＇-UTR）. Phylogenetic analysis on nucleotide （nt） sequences indicated that ACLSV isolates obtained were clustered into two branches or sub-branches in the subgroup I-1 of the group I. The ACLSV isolate hz48 from sand pear in Hangzhou showed low identities with the other isolates from China, ranging from 83.5% to 87.5% （nt） and 82.6% to 86.2% （ amino acid, aa）, and was grouped into a separate sub-branch of sub-group I-1 in the phylogenetic tree. The aa multiple alignment of sequenced partial CP revealed that ACLSV isolates showed high variability at the N-terminal part of CP region overlapped with the MP region and relatively conserved at the C-terminal part, whereas the variation positions were also presented in C-terminal part of CP region from the isolate hz48 analyzed in this study.

关 键 词：苹果褪绿叶斑病毒 序列分析 非翻译区 CP基因 砂梨 分离物 ACLSV 核果类果树 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]