复方丹参注射液对人羊膜上皮细胞水通道蛋白3表达的影响  

Effects of Compound Salvia miltiorrhiza Injection on Aquaporin 3 Expression in Human Amniotic Epithelial Cells

作　　者：张译文[1] 马小燕[1] 丁盛娣[1] 谢爱兰[1] 朱雪琼[1] 

机构地区：[1]浙江省温州医学院附属第二医院妇产科,浙江温州325027

出　　处：《中国中西医结合杂志》2013年第1期51-55,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省中医药管理局重点项目(No.2010ZZ009);温州市对外合作交流科技计划项目(No.H20100067)

摘　　要：目的研究复方丹参注射液对人羊膜上皮细胞(human amniotic epithelial cells,hAECs)水通道蛋白3(aquaporin 3,AQP3)表达的影响,探讨复方丹参注射液治疗羊水量过少的机制。方法选取无并发症的8例羊水过少和8名羊水量正常的足月产妇的hAECs进行原代培养,采用RT-PCR和免疫印迹Western blot技术,检测复方丹参注射液不同浓度(0.000、0.001、0.010、0.020、0.060、0.100mg/mL)、不同作用时间(0、6、12、24、48h)作用前后,两组hAECs中AQP3 mRNA和蛋白的表达。结果 (1)羊水过少组hAECs中AQP3的表达较羊水正常组下调(P<0.05)。(2)复方丹参注射液浓度为0.010mg/mL时,两组hAECs中AQP3的表达均达到峰值,与其他浓度作用结果比较,差异有统计学意义(P<0.05)。(3)0.010mg/mL浓度的复方丹参注射液作用12h,两组hAECs中AQP3的表达均达到峰值,与其他作用时间比较,差异有统计学意义(P<0.05)。(4)0.010mg/mL浓度的复方丹参注射液作用12h后,两组hAECs中AQP3的表达均上调,但羊水过少组中AQP3的表达上调较羊水正常组明显(P<0.05)。结论复方丹参注射液可调节hAECs中AQP3的表达,羊水过少时复方丹参注射液对hAECs中AQP3表达的调节作用更加明显。Objective To study the effects of Compound Salvia miltiorrhiza Injection （CSI）on aquaporin 3 （AQP3）expression in human amniotic epithelial cells （hAECs）, and to explore its mechanisms for treating oligohydramnios. Methods The hAECs selected from 8 human term pregnancies with oligohydramnios and no other complications （as the test group）and 8 human term pregnancies with normal amniotic fluid volume （as the control group）were primarily cultured. The mRNA and protein expressions of AQP3 in hAECs were detected using reverse transcription-polymerase chain reaction and Western blot with various concentrations of CSI （0.000, 0.001, 0.010, 0.020, 0.060, and 0. 100 mg/mL, respectively）at different time points （0, 6, 12, 24, and 48 h, respectively）. Results （1）Compared with the control group, the AQP3 expression was down-regulated in the test group （P 〈0.05）. （2）The AQP3 expression in the two groups reached the peak when the concentration of CSI was 0. 010 mg/mL, showing statistical difference when compared with other concentrations （P 〈 0.05）. （3）The AQP3 expression reached the peak when 0.010 mg/mL CSI acted for 12 h, showing statistical difference when compared with other concentrations （P 〈0.05）. （4）The AQP3 expression was up-regulated in the two groups when 0.010 mg/mL CSI acted for 12 h. But the up-regulated AQP3 expression was more obvious in the test group than in the control group （P 〈0.05）. Conclusions CSI could regulate the AQP3 expression in hAECs. CSI showed more obvious effects on the AQP3 expression in hAECs of oligohydramnios human term pregnancies.

关 键 词：水通道蛋白3 羊水过少 人羊膜上皮细胞 复方丹参注射液 

分 类 号：R285.5[医药卫生—中药学]