胃乐散对大鼠胃溃疡黏膜碳酸氢盐转运蛋白CFTR、SLC26A3及SLC26A6的作用  

Effects of Weile Powder on Bicarbonate Transporters CFTR SLC26A3 and SLC26A6 in Gastric Ulcers of Rats

作　　者：文国容[1] 徐靖宇[1] 刘雪梅[1] 赵正兰[1] 江义霞[1] 谢睿[1] 陈萍[2] 余丽梅[3] 庹必光[1] 

机构地区：[1]遵义医学院附属医院消化内科,贵州遵义563000 [2]遵义医学院附属医院药剂科,贵州遵义563000 [3]遵义医学院贵州省细胞工程重点实验室,贵州遵义563000

出　　处：《中国中西医结合杂志》2013年第1期81-84,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30860092);贵州省中医药管理局基金资助项目[黔中医药发(2009)79号]

摘　　要：目的研究胃乐散对大鼠胃溃疡黏膜碳酸氢盐分泌的影响,并探讨其作用机制。方法 SD大鼠48只,随机分为正常对照组,模型组,胃乐散低剂量组[0.075g/(mL·kg)]、中剂量组[0.150g/(mL·kg)]、高剂量组[0.300g/(mL·kg)]和雷尼替丁组[0.030g/(mL·kg)],每组8只。采用冰醋酸烧灼法制备大鼠胃溃疡模型,制模后第2天,各用药组开始给药,连续用药后第14天处死大鼠,取溃疡部位组织提取蛋白,用免疫印迹法(Western blot)检测组织Cl-/HCO-3离子交换体solute carrier26A3(SLC26A3)及solute carrier26A6(SLC26A6)的含量;将大鼠胃溃疡及周围组织切片,用免疫荧光法观察囊性纤维化跨膜转运调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)的变化。结果与模型组比较,用药后胃乐散高剂量组及雷尼替丁组大鼠胃黏膜中SLC26A3的表达增加,差异有统计学意义(P<0.05);雷尼替丁组,胃乐散中、高剂量组大鼠胃黏膜中SLC26A6的表达均增加,差异有统计学意义(P<0.05,P<0.01);胃乐散中、高剂量组大鼠胃溃疡黏膜的CFTR表达也明显增加,差异有显著统计学意义(P<0.01)。结论胃乐散能提高大鼠胃溃疡黏膜SLC26A3、SLC26A6和CFTR的表达,增加碳酸氢盐的分泌,保护胃黏膜。Objective To investigate the effects of Weile Powder （WLP）on bicarbonate transporters in rats with gastric ulcers, and to probe its functional mechanisms. Methods The 48 SD rats were randomly divided into the normal control group, the model group, the low dose WLP group （at the daily dose of 0.075 g/mL）, the middle dose WLP group （at the daily dose of 0. 150 g/mL）, the high dose WLP group （at the daily dose of 0.030 g/mL）, and the ranitidine group （at the daily dose of 0.030 g/mL）, 8 in each group. The gastric ulcer rat model was prepared by the glacial acetic acid cauterization method. Rats in each medication group were administered from the 2nd day of modeling. Rats were sacrificed after 14-day successive medication. The protein was extracted from the ulcer tissue. The protein expressions of solute carrier26A3 （SLC26A3）and solute carrier26A6 （SLC26A6）were detected using Western blot. The gastric ulcer and its peripheral tissue were sectioned. The changes of cystic fibrosis transmembrane conductance regulator （CFTR）were measured by immunofluorescence. Results Compared with the model control group, the expression levels of SLC26A3 increased in the high dose WLP group and the ranitidine group with statistical difference （P 〈0.05）. The expression levels of SLC26A6 increased in the high and middle dose WLP groups and the ranitidine group with statistical difference （P 〈0.05）. The expression level of CFTR also obviously increased in the high and middle dose WLP groups （P 〈0.01 ）. Conclusion WLP could elevate the expression levels of SLC26A6, SLC26A3, and CFTR, increase the secretion of bicarbonate, thus protecting the gastric mucosa.

关 键 词：胃乐散 胃溃疡 离子交换体26A3 离子交换体26A6 囊性纤维化跨膜转运调节因子 免疫荧光法 免疫印迹法 

分 类 号：R285.5[医药卫生—中药学]