菊苣多糖对糖尿病大鼠心肌缺血再灌注过氧化的影响  

The protective effect of chicory polysaccharides on diabetic-resulted myocardial ischemia-reperfusion injury in rats

作　　者：李屹[1] 刘晓舒[1] 杨鑫[1] 马越娇[1] 张建军[1] 车光昇[1] 宋光熠[1] 

机构地区：[1]辽宁省基础医学研究所,辽宁沈阳110101

出　　处：《中国老年学杂志》2013年第2期330-331,共2页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.30873257)

摘　　要：目的探讨菊苣多糖对链脲菌素糖尿病大鼠心肌缺血再灌注损伤过氧化的影响。方法应用链脲菌素复制大鼠糖尿病模型,12 w后,腹腔注射戊巴比妥钠麻醉下气管插管安装呼吸机,开胸结扎冠状动脉左室降支60 min后松开结扎线再灌注60 min。标Ⅱ导联全程记录心电图,并于再灌注60 min取血、心脏。检测肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)。结果菊苣多糖组的CK、LDH、MDA水平明显低于糖尿病组,SOD、GSH-PX活性高于糖尿病组(均P<0.05)。结论菊苣多糖通过抗过氧化作用对链脲菌素糖尿病大鼠心肌缺血再灌注损伤具有保护作用。Objective To observe the protective effect of chicory polysaccharides on myocardial ischemia-reperfusion injury resulted by streptozotocin diabetic in rats. Methods Streptozotocin intraperitoneal was used to induce diabete（DM） rat model. After 12-week injec- tion, DM rats were anesthetized with pentobarbital, TI and breathing machine installed. Then DM rats were ligated descending branch of CA on LV for 60 minutes, and loosed the ligation to realize the reperfusion for 60 min. Heart and blood were collected for testing CK, LDH, MDA, SOD, and GSH-PX. Results CK, LDH and MDA in Chicory Polysaccharides group were significantly lower than those of DM group, however, the activities of SOD and GSH-Px turned out just opposite. Conclusions Chicory polysaccharide definitely has a protective effect to the diabetic-resulting myocardial ischemia-reperfusion injury in rat.

关 键 词：菊苣多糖 糖尿病 心肌缺血再灌注 抗过氧化作用 

分 类 号：R587.1[医药卫生—内分泌]