去冷沉淀血浆的质量分析  

Analysis of quality of cryoprecipitate-reduced plasma

作　　者：马莉[1] 孙盼[1] 林方昭[1] 刁戈[1] 李剑平 张红抑[3] 刘建强[4] 张心声[5] 柏则蓉 周静宇[7] 黎美君 李长清[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]辽宁省血液中心,辽宁沈阳110044 [3]昆明血液中心,云南昆明650106 [4]陕西省血液中心,陕西西安710061 [5]山东省血液中心,山东济南250014 [6]盐城市中心血站,江苏盐城224005 [7]江苏省血液中心,江苏南京210042 [8]重庆血液中心,重庆400015

出　　处：《中国生物制品学杂志》2013年第1期81-83,共3页Chinese Journal of Biologicals

基　　金：国家卫生公益性行业科研专项(200902008)

摘　　要：目的比较去冷沉淀血浆(Cryoprecipitate-reduced plasma,CRP)中系列凝血因子、纤维蛋白原(Fibrinogen,Fib)和血管性血友病因子裂解蛋白酶(A disintegrin-like and metalloprotease with thrombospondin-1 repeats 13,ADAMTS-13)水平的变化。方法用140人份新鲜冰冻血浆(Fresh frozen plasma,FFP)制备CRP,采用Biggers一期法测定FFP和CRP中FⅡ、FⅤ、FⅦ、FⅧ、FⅨ、FⅩ、FⅪ、FⅫ的促凝活性;免疫比浊法测定Fib和血管性血友病因子抗原因子活动度(vWF∶Ag)水平;荧光共振能量转移法(Fluorescence resonance energy transfer,FRET)测定70人份FFP和CRP中的ADAMTS13活性及抗原含量。结果与FFP比较,CRP中FⅧ∶C、FⅤ∶C、FⅦ∶C、FⅨ∶C、FⅩ∶C、FⅪ∶C、Fib、vWF∶Ag水平均明显下降,且差异均有统计学意义(P<0.05或<0.001);FⅡ∶C、FⅫ∶C、ADAMTS13抗原含量和活性差异均无统计学意义(P>0.05)。结论 CRP可代替FFP用于血栓性血小板减少性紫癜(Thrombotic throm-bocytopenic purpura,TTP)的治疗,而不适用于FⅧ、Fib及vWF缺乏患者的补充治疗。Objective To compare the levels of coagulation factors,fibrinogen（Fib） and Von Willebrand factor cleaving protease,a disintegrin-like and metalloprotease with thrombospondin-1 repeats 13（ADAMTS13）,in cryoprecipitate-reduced plasma（CRP）.Methods CRP was prepared with the fresh frozen plasma（FFP） from 140 donors.The coagulation-promoting activities of FⅡ,FⅣ,FⅦ,FⅧ,FⅨ,FⅩ,FⅪ and FⅫ in FFP and CRP were determined by one-stage Biggers method,while the activities of Fib and Von Willebrand factor antigen（vWF ∶ Ag） by immune turbidimetry.The activity and antigen contents of ADAMTS13 in FFP and CRP were determined by fluorescence resonance energy transfer（FRET） assay.Results As compared with those in FFP,the levels of FⅧ ∶ C,FⅤ ∶ C,FⅦ ∶ C,FⅨ ∶ C,FⅩ ∶ C,FⅪ ∶ C,Fib,vWF ∶ Ag decreased significantly（P 0.05 or P 0.001）,while the antigen content and activity of FⅡ ∶ C,FⅫ ∶ C and ADAMTS13 showed no significant difference（P 0.05）.Conclusion CRP may be used for treatment of thrombotic thrombocytopenic purpura（TTP） instead of CRP,while is unsuitable for complementary treatment of FⅧ,Fib and vWF deficiency.

关 键 词：去冷沉淀血浆 凝血因子 纤维蛋白原 血管性血友病因子裂解蛋白酶 血栓性血小板减少性紫癜 

分 类 号：R457.14[医药卫生—治疗学] R392-33[医药卫生—临床医学]