亚单位流感疫苗中裂解剂壬苯醇醚-9含量两种检测方法的比较  

Determination of content of nonoxynol-9 as a cleavage reagent in influenza virus subunit vaccine by two methods

作　　者：田文莉 杨江山 万亚芬 祁骥 杨旭 

机构地区：[1]天士力金纳生物技术(天津)有限公司,天津300410

出　　处：《中国生物制品学杂志》2013年第1期120-121,共2页Chinese Journal of Biologicals

摘　　要：目的比较高效液相色谱法(HPLC)和紫外分光光度法检测亚单位流感疫苗中裂解剂壬苯醇醚-9含量的效果。方法分别采用HPLC法和紫外分光度法检测亚单位流感疫苗中间体和成品中壬苯醇醚-9的含量,并取中间体进行SDS-PAGE分析。结果紫外分光光度法测定亚单位流感疫苗成品和中间体的壬苯醇醚-9含量比HPLC法高10%以上,测定中间体3壬苯醇醚-9含量的结果差异更显著;SDS-PAGE分析显示中间体1、2、3的核蛋白(NP)含量差异显著,大多数存在于中间体3中。结论 HPLC法可代替紫外分光光度法检测亚单位流感疫苗中裂解剂壬苯醇醚-9的含量。Objective To compare the determination results of content of nonoxynol-9 as a cleavage reagent in influenza virus subunit vaccine by HPLC and UV spectrophotometry.Methods The nonoxynol-9 contents in intermediate and final product of influenza virus subunit vaccine were determined by HPLC and UV spectrophotometry separately,and the intermediate was analyzed by SDS-PAGE.Results The nonoxynol-9 contents in intermediate and final product,especially in intermediate,of influenza virus subunit vaccine determined by UV spectrophotometry increased by more than 10% as compared with those by HPLC.SDS-PAGE showed the nucleoprotein（NP） contents were significantly different in intermediates 1,2 and 3,which was the highest in intermediate 3.Conclusion HPLC may be used for determination of nonoxynol-9 content in influenza virus subunit vaccine instead of UV spectrophotometry.

关 键 词：亚单位流感疫苗 壬苯醇醚-9 高效液相色谱法 紫外分光光度法 

分 类 号：O656.9[理学—分析化学]