膜荚黄芪肉桂酸-4-羟化酶(C4H)基因克隆与序列分析  

Cloning and sequence analysis of cinnamate acid-4-hydroxylase(C4H) gene from Astragalus membranaceus

作　　者：王树斌[1] 全雪丽[1] 具红光[1] 朴世领[1] 吴松权[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《延边大学农学学报》2012年第4期277-281,323,共6页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金(30860036);吉林省自然科学基金(201115228)

摘　　要：肉桂酸-4-羟基化酶是苯丙烷途径中继L-苯丙氨酸解氨酶(PAL)之后的第2个关键酶。为了从分子水平上了解C4H基因的功能特性,本文从植物进化的角度出发,根据Genebank中已有的豆科植物C4H氨基酸保守序列为基础,设计引物,通过RT-PCR和cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE)方法克隆膜荚黄芪C4H基因,对其序列进行分析,为进一步研究其功能奠定基础。Cinnamic acid-4-hydroxylase（C4H,EC1.14. 13.11）is the second key enzyme in phenylpropanoid metabolic pathway after L-phenylalnine ammonialyase（PAL）. In order to understand the functional prop- erties of C4H gene, designed PCR primers that target evolutionarily conserved sequeces of C4H gene in plants and cloned full-length sequences of C4H gene in A. membranaceus by the technique of RT-PCR and RACE, analysised characteristic of sequence.

关 键 词：膜荚黄芪 C4H 基因克隆 序列分析 

分 类 号：S567[农业科学—中草药栽培]