固相萃取-高效液相色谱法检测水中多种拟除虫菊酯类农药  

Determination of Five Pyrethroids in Water Using Solid Phase Extraction High Performance Liquid Chromatography

作　　者：沈登辉[1] 单晓梅[1,2] 陆蓓蓓[1] 谢继安[2] 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生学系,安徽合肥230032 [2]安徽省疾病预防控制中心,安徽合肥230601

出　　处：《环境与职业医学》2013年第1期49-52,共4页Journal of Environmental and Occupational Medicine

基　　金：安徽省自然科学基金资助项目(编号:11040606M216)

摘　　要：[目的]研究同时测定水中甲氰菊酯、氯氟氰菊酯、溴氰菊酯、氰戊菊酯、氯菊酯5种拟除虫菊酯类农药污染物的固相萃取-高效液相色谱方法。[方法]水样经C18小柱富集,以异丙醇洗脱,采用光电二极管阵列检测器检测,利用C18色谱柱分离,以乙腈溶液和纯水为流动相等梯度洗脱,流速1.0mL/min,在波长205nm进行测定,外标法定量。[结果]5种拟除虫菊酯类农药在0.0～5.0mg/L浓度范围内具有良好的线性关系,方法最低检测质量浓度为0.025～0.050μg/L,回收率范围为88.0%～102.2%,相对标准偏差为1.0%～3.8%。[结论]该固相萃取-高效液相色谱法灵敏、准确,适用于水源水及生活饮用水中痕量拟除虫菊酯类农药残留的检测。[ Objective ] To establish a method for the simultaneous determination of 5 pyrethroids in source water or drinking water, i.e. fenpropathrin, cyhalothrin, dehamethrin, fenvalerate, and permethrin, using solid phase extraction combined with high performance liquid chromatography. [ Methods ] The water samples were extracted and condensed with solid-phase extraction using C18 cartridges and eluted by isopropanol. Separation was carried out on a Waters Sunfire C18 column, using acetonitrile and water as mobile phase gradient model at a flow rate of 1.0 mL/min, with detective wavelength at 205 nm. Quantification was calculated by an external standard method. [ Results ] The linear range of proposed determination methodology was 0.0-5.0 mg/L. The minimum detectable concentration was 0.025-0.050 ~g/L. The recoveries were between 88.0% and 102.2% with a relative standard deviation in the range of 1.0%-3.8%. [ Conclusion ] The method is sensitive, accurate, and applicable to detect the trace pyrethroid residues in source water and drinking water.

关 键 词：固相萃取 高效液相色谱 拟除虫菊酯农药 

分 类 号：R115[医药卫生—公共卫生与预防医学]