小分子重编程诱导肝上皮样干细胞为胰岛素分泌细胞的研究  

Induction of hepatic epithelial-like stem cells into insulin producing cells by reprogramming defined small molecules

作　　者：王洪刚 刘艳梅[3] 马玉珍 刘建萍[3] 林季[4] 申晶[4] 郑郁[4] 程愈[4] 郝好杰[4] 韩为东[4] 母义明[4] 王清莲 

机构地区：[1]内蒙古自治区人民医院科研处,呼和浩特010017 [2]内蒙古自治区人民医院生殖中心,呼和浩特010017 [3]江西省人民医院干部保健科 [4]中国人民解放军总医院内分泌科

出　　处：《中华内分泌代谢杂志》2013年第1期79-82,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：内蒙古自然科学基金重大项目（20090505）;国家高技术研究发展计划（863计划,2011AA020113）

摘　　要：采用阶段诱导法进行体外诱导肝上皮样干细胞（WB—F344）为胰岛素分泌细胞（IPCs），将IPCs移植至糖尿病大鼠体内，监测血糖变化。结果显示，RT—PCR和免疫荧光技术分别检测到IPCs中表达胰岛β细胞的特异性转录因子及蛋白。IPCs移植糖尿病大鼠体内后，明显改善大鼠的高血糖状态。提示肝上皮样干细胞可以在体外经小分子重编程为有功能的胰岛素分泌细胞，其有助于糖尿病的治疗。Using phase induction in vitro to induce hepatic epithelial-like stem cells （ WB-F344 ） into insulin producing cells （ IPCs ） , the IPCs were transplanted into diabetic rats, and the blood glucose level was monitored. The results of RT-PCR and immunofluorescence showed that genes and proteins of pancreatic islet beta cell and phenotypic markers were successfully detected in the IPCs, which significantly improved hyperglycemia after being transplantated into diabetic rats. These results suggest that hepatic epithelial-like stem cells in vitro can be induced into functional insulin producing cells by small molecules and that this finding may contribute to the treatment of diabetes.

关 键 词：肝上皮样干细胞 小分子 胰岛素分泌细胞 重编程 糖尿病 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]