促黑素对破骨细胞形成的影响  

Effect of α-MSH on osteoclast formation

作　　者：刘欣[1] 林良妍[1] 赵霞[1] 乔珍[1] 綦才辉[1] 金勇君[1] 

机构地区：[1]山东省滨州医学院烟台附属医院内分泌科,,264100

出　　处：《中华内分泌代谢杂志》2013年第1期83-85,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30660070）;山东省中青年科学家科研奖励基金资助项目（BS2010YY002）

摘　　要：以核因子κB受体活化因子配体（RANKL）和促黑素（α-MSH）孵育Raw264．7细胞6d，经抗酒石酸酸性磷酸酶染色观察破骨细胞形成的数目．并检测酸性磷酸酶活性。RT-PCR检测Raw264．7细胞5种黑皮质素受体（MCR）的表达。结果显示，与RANKL组相比较，RANKL＋α—MSH组显著增加了破骨细胞的形成数量（P〈0．05），呈剂量依赖性，单独应用α—MSH组未见破骨细胞形成。RANKL组和RANKL＋α-MSH组的酸性磷酸酶活性显著高于对照组和α-MSH组（P〈0．05），但前2组之间差异无统计学意义（P〉0．05）；RT—PCR结果显示Raw264．7细胞5种MCR均表达。结果提示，α-MSH可能通过RANK信号通路促进破骨细胞形成。Raw264.7 cells were incubated with receptor activator of NV-kappa B ligand （RANKL） and α- melanocyte stimulating hormone（α-MSH） for6 d. The amount of osteoclast cells were counted by tartrate resistant acid phosphatase staining and the acid phosphatase activity was assayed. The expressions of 5 melanocortin receptors （MCR） in Raw264.7 cells were determined by RT-PCR. The results showed that the number of osteoclasts in RANKL ＋α-MSH group was significantly increased compared with RANKL group （P 〈 0.05 ）, but there was no osteoclast formation in α-MSH group. Compared with control group and α-MSH group, the acid phosphatase activities were significantly increased in RANKL group and α-MSH＋RANKL group （ P〈0.05 ）. All five MCRs were expressed in the Raw264.7 cells shown by RT-PCR. These results suggest that α-MSH may promote osteoclasts formation through RANK signaling pathway.

关 键 词：促黑素 破骨细胞形成 黑皮质素受体 

分 类 号：R580[医药卫生—内分泌]