Osterix在调控脊柱发育中的作用  

Role of Osterix in development of spine in mice

作　　者：宗兆文[1] 陈思旭[1] 贾敏[1] 沈岳[1] 赵玉峰[1] 郭庆山[1] 华翔[1] Jerry Feng 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤科,创伤、烧伤与复合伤国家重点实验室,重庆400042 [2]德克萨斯州卫生科学中心Baylor医学院,美国达拉斯75246

出　　处：《第三军医大学学报》2013年第3期220-222,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81271935);“十二五”科研项目重大项目分课题(AWS11J008);伤、烧伤与复合伤国家重点实验室自主课题(SKLZZ201124)~~

摘　　要：目的观察核转录因子Osterix在脊柱发育中的作用。方法采用Lox P/Cre系统,以2.3 kbⅠ型胶原蛋白为启动子,在小鼠成骨细胞和骨细胞中特异性敲除Osterix,观察Osterix敲除小鼠大体情况,采用X线摄片、HE染色和Nissl染色观察其对小鼠脊柱发育的影响。结果 Osterix敲除小鼠后肢瘫痪,行走时前肢运动正常,拖动后肢爬行,Osterix的敲除效率约为75%;X线片结果显示,Osterix敲除小鼠形体变小,胸腰段脊柱出现楔形椎体、严重的脊柱侧弯和椎管狭窄等畸形;HE染色结果显示胸腰段脊椎结构发育严重异常;Nissl染色结果显示,受累节段脊髓神经元结构严重受损。结论 Osterix在脊柱发育中起到重要作用,敲除后可导致脊柱侧弯等畸形。Object ve To investigate the role of Osterix in spine development. Methods Osterix was knocked out by crossing 2.3 kb Collagen I Cre mice with Osterix Lox P ＋/＋ mice. X-ray radiology, HE staining and Nissl staining were employed to observe the effect of Osterix knockout on spine development. Results Osterix knockout mice demonstrated short stature, wedged vertebrae, severe scoliosis and spinal stenosis at the thoracic and lumbar regions of spine. HE staining showed that the structure of growth plate, vertebral body and spinal canal were totally disorganized. Nissl staining revealed that the neurons in the spinal cord at the inflicted region were strikingly development, and Osterix knockout leads to damaged. Conclusion Osterix plays an important role in spine severe spinal deformities such as scoliosis.

关 键 词：OSTERIX 脊柱 发育 基因敲除 

分 类 号：R336[医药卫生—人体生理学] R394[医药卫生—基础医学]