钩藤总碱对焦虑模型行为学和脑组织单胺类神经递质的影响  

Effect of rhynchophylla total alkaloids on behavior and contents of monoamine neurotransmitters in brain tissues of anxiety model rats

作　　者：李晶晶[1] 汤建林[1] 胡岚岚[1] 周世文[1] 周吉银[1] 

机构地区：[1]第三军医大学新桥医院临床药理基地,重庆400037

出　　处：《第三军医大学学报》2013年第3期237-240,共4页Journal of Third Military Medical University

摘　　要：目的考察钩藤总碱(rhynchophylla total alkaloids,RTA)抗焦虑药效。方法采用国际通用的大鼠高架十字迷宫焦虑动物模型(elevated plus-maze anxiety animal model,EPM)和小鼠期待性焦虑实验,观察RTAⅠ组[200 mg/(kg.d)]、RTAⅡ组[400 mg/(kg.d)]、RTAⅢ组[800 mg/(kg.d)]3个剂量组对大、小鼠焦虑行为学的影响;采用酶联免疫吸附法(ELISA)测定RTA干预前后EPM大鼠的脑组织内去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)单胺类神经递质的含量。结果与空白对照组比较,RTA 3个剂量组大鼠进入开放臂次数比例(OE%)和在开放臂滞留时间比例(OT%)有显著升高(P<0.05),RTA 3个剂量组小鼠均无应激性体温升高,RTAⅢ组EPM大鼠脑组织中NE、DA、5-HT含量较空白对照组显著下降(P<0.05)。结论在大鼠EPM和小鼠期待性焦虑实验中,RTA显示出一定的抗焦虑作用,其作用机制可能与降低EPM刺激后大鼠脑组织中NE、DA和5-HT含量有关。Objective To investigate the putative anxiolytic effect of rhynchophylla total alkaloids （RTA）. Methods The internationally accepted elevated plus-maze anxiety animal model （EPM） in rats and the anticipatory anxiety experiment in mice were performed to observe the effects of RTA I [ 200 rag/（ kg · d） ], RTAIIE400 mg/（kg · d）] and RTAm[800 mg/（kg · d）l on the behavior of rats and mice. Then the contents of monoamine neurotransmitters including norepinephrine （NE）, dopamine （DA） and 5- hydroxytryptamine （5-HT） in rat brain tissues were determined by enzyme-linked immunosorbent assay （ELISA）. Results Compared with the control group, the percentage of the times of rats entering the open arm （ OE% ） and percentage of time spent in the open arm （ OT% ） were significantly higher （P 〈 0.05 ） in all the RTA treatment groups. There was no stress-induced hyperthermia （SIH） in the mice of three RTA groups. The contents of NE, DA and 5-HT in rat brain tissues decreased significantly in all the RTA groups as compared with the control group （P 〈 0.05 ）. Conclusion According to the rat EMP model and mice anticipatory anxi- ety experiment, RTA shows certain anxiolytic effect, and the mechanism may be related to the reduction of NE, DA and 5-HT in the brain tissues of EMP rats.

关 键 词：钩藤总碱 高架十字迷宫模型 小鼠期待性焦虑实验 单胺神经递质 抗焦虑 

分 类 号：R285.5[医药卫生—中药学] R338.14[医药卫生—中医学]