PGC-1,NRF-2α和mtTFA在视觉剥夺大鼠视皮质内的表达下调  

Down expressions of PGC-1,NRF-2α and mtTFA in the rat visual cortex after visual deprivation

作　　者：陈翔[1] 赵晓[2] 

机构地区：[1]解放军总医院眼科,北京100853 [2]解放军总医院肿瘤内科,北京100853

出　　处：《基础医学与临床》2013年第2期215-218,共4页Basic and Clinical Medicine

摘　　要：目的观察核呼吸因子-2α(NRF-2α)在正常发育及视觉剥夺大鼠视皮质内的表达。方法构建视觉剥夺大鼠模型,利用半定量PCR和免疫印迹的方法检测过氧化物酶体增生激活受体γ协同刺激因子(PGC-1)、NRF-2α和线粒体转录因子A(mtTFA)在视皮质内的表达。结果视觉剥夺大鼠视皮质内PGC-1、NRF-2α和mtTFA表达较正常组明显降低(P<0.05)。结论 NRF-2α的基因转录及蛋白表达水平依赖于正常视觉信号的刺激,异常的视觉经验使视皮质神经元兴奋性降低,造成NRF-2α表达的明显下调。Objective Observe nuclear respiratory factor-2a （NRF-2a） expression in the rat visual cortex in the nor- real development and visual deprivation. Methods To develope visual deprivation rat model using quantitative PCR and Western blot detection PPAR y coactivator-1 （PGC-1）, NRF-2ot and mitochondrial transcription factor （mtTFA） expressed in the rat visual cortex. Results Data shows that of visual deprivation rat model resulted in marked reduc- tion of NRFs＇ RNA and protein expression （P 〈0. 05）. Conclusion NRF-2a gene transcription and protein expres- sion level depend on the stimulation of normal visual much influence on mitochondria function,resulting in signal, Our results proved evidence that visual deprivation has a significantly down-regulated exoression of NRF-2a.

关 键 词：核呼吸因子-2α 视觉剥夺 视皮质 

分 类 号：R473.77[医药卫生—护理学]