‘津田’芜菁光形态建成抑制因子COP1蛋白cDNA的克隆及表达分析  

cDNA Cloning and mRNA Expression of Photomorphogenic Negative Regulator COP1 in‘Tsuda’Turnip(Brassica rapa L. ssp. rapifera)

作　　者：孙梅[1] 周波[1] 王宇[1] 刘明雪[1] 安春鹏[1] 李玉花[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《园艺学报》2013年第1期79-88,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30730078)

摘　　要：通过 RT-PCR 和 RACE 的方法得到了编码‘津田’芜菁(Brassica rapa L. ssp. rapifera‘Tsuda’)BrCOP1 的 cDNA 序列,长 2 034 bp,编码 677 个氨基酸,与拟南芥同源性达 94%,命名为 BrCOP1,GeneBank收录号为 JF738111。Northern 杂交分析表明 BrCOP1 的表达没有组织特异性,而在整株幼苗及成熟植株肉质根表皮中受长波紫外线(UV-A)诱导表达,但成熟植株的直根表皮中表达量不随处理时间延长而增加。这暗示 BrCOP1 作为光控发育的重要调控因子,可能在 UV-A 诱导‘津田’芜菁花青素合成及光信号转导中起重要作用。The COP1 gene（BrCOP1，accession number JF738111）was isolated from total RNA of Brassica rapa L. ssp. rapifera‘Tsuda’by RT-PCR and RACE. BrCOP1 cDNA contained an opening reading frame of 2 034 bp，encoding a predicted protein of 677 amino acid residues. The deduced amino acid sequence of BrCOP1 shared 94% identity with Arabidopsis thaliana COP1. Northern blotting results demonstrated that expression of BrCOP1 exhibited no tissue specificity，and had no obvious difference in seedlings under various treatments but was induced by UV-A light，while the expression of BrCOP1 was induced after exposed to UV-A for 6 hours in the swollen hypocotyls，however expression level was not increased with the prolongation of time. The above results suggested that BrCOP1，as a key factor of light-dependent development，might play a crucial role in UV-A induced anthocyanin biosynthesis and light signal transduction.

关 键 词：芜菁 COP1 克隆 表达特性 

分 类 号：S631.3[农业科学—蔬菜学]